We narrowed to 22,168 results for: LEA
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pVoPo-mSc
Plasmid#187842PurposeConstitutive mScarlet-I-expression vector for Fusobacterium nucleatumDepositorInsertmScarlet-I
ExpressionBacterialAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
ExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mOrange LIC cloning vector (MBP-mOrange)
Plasmid#29748DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-AKAP95-FRB
Plasmid#138254PurposeFull-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.DepositorInsertAKAP95-FRB (AKAP8 Human)
TagsFRB rapamycin binding domain and HA epitopeExpressionMammalianPromoterCMVAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro
Plasmid#205631PurposeExpression of TnpBmax in mammalian cellsDepositorInsertISDra2-TnpB optimized for mammalian expression
TagsNLS-3xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro_shTBK1_3185
Plasmid#167294PurposeLentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.DepositorAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
TagsmKate2ExpressionBacterialAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
ECFP-Nucleus-7
Plasmid#55346PurposeLocalization: SV40 NLS Triplet, Excitation: 433, Emission: 475DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
TagsECFP-NLSExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-CMVSP6-nEGFP-SV40-PURO
Plasmid#138364PurposeLentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selectionDepositorInsertnuclear EGFP
UseLentiviralExpressionMammalianAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_NES_C1
Plasmid#85062PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x6
Plasmid#58770PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AF20-double-tag
Plasmid#108389PurposeExpress human PC1DepositorInsertPKD1 (PKD1 Human)
TagseGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5ExpressionMammalianPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-deSpCas9
Plasmid#92114PurposeExpression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)DepositorInsertdead/inactive eSpCas9 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, H840A, K848A, K1003A, R1060APromoterCbhAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN42
Plasmid#61345PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS10 and PKIt NESExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Split Cas9 D10A Nickase with GyrA intein
Plasmid#58695PurposeExpresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase productionDepositorInsertD10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
UseAAV and CRISPRTagsGyrA Nsplit Intein, HA Tag, and NLSExpressionMammalianMutationD10A nickase converting mutation to SpCas9PromoterCBhAvailable SinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
7TFP CDH1 mutant reporter
Plasmid#91703Purposelentiviral luciferase reporter containing CDH1 promoter with mutant E-boxesDepositorInsertCDH1 mutant promoter (CDH1 Human)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationmutant E-boxesPromoterCDH1 mutantAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pclbw-opa1(isoform 7DeltaS1)-myc
Plasmid#62846PurposeMammalian Expression of myc-tagged Opa1 isoform 7 Delta S1DepositorInsertmmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)
UseRetroviralTags9xMycExpressionMammalianPromotercmv-bactinAvailable SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NBid-PhoCl2c-CBid
Plasmid#164051PurposeOptogenetic manipulation of cell apoptosis using PhoCl2cDepositorInsertNBid-PhoCl2c-CBid
Tagskozak sequenceExpressionMammalianPromoterCMV promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ EB3-mKate2
Plasmid#105940Purposein vitro transcriptionDepositorAvailable SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only
