We narrowed to 56,206 results for: NAM

1. pAM5085

   Plasmid

   #85122

   Purpose

   PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.

   Depositor

   Susan Golden

   Article

   Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

   Insert

   PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
   

   Tags

   ECFP

   Expression

   Bacterial

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEX-Antenna-GDI Cdc42

   Plasmid

   #101681

   Purpose

   "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

   Depositor

   Klaus Hahn

   Article

   Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

   Insert

   Cdc42
   

   Tags

   6xHis and FRET Antenna (mCerulean-cpVenus)

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWPI-IRES-eGFP-Mefv iso1

   Plasmid

   #92104

   Purpose

   Expression in eukaryotic cells and generating stable cell lines

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   Mefv isoform1 (Mefv Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MO70-mJP-4

   Plasmid

   #79598

   Purpose

   Transient and retroviral expression

   Depositor

   Yousang Gwack

   Article

   Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

   Insert

   Junctophilin-4 (Jph4 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pB-RTP2-Integration

   Plasmid

   #129459

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP2 (RTP2 Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE (Tet-On)

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

   Plasmid

   #178985

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

   Plasmid

   #202617

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   FRT-JEDI-1P-Kv
   

   Use

   AAV; Flp/frt

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKII-ASAP2s

   Plasmid

   #101275

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAMKIIa

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover

   Plasmid

   #124549

   Purpose

   Lentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)

   Depositor

   Markus Covert

   Article

   Lane et al Cell Syst. 2017 Apr 26;4(4):458-469.e5.

   Insert

   p65 promoter- p65 CDS-Clover
   

   Use

   Lentiviral

   Tags

   Clover

   Expression

   Mammalian

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-AMPKsub-YPet-NES

    Plasmid

    #84635

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mCherry-LKB1(KD)

    Plasmid

    #84640

    Purpose

    mCherry-tagged, LKB1(K78I); kinase-dead mutant

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(K78I) (STK11 Synthetic, Human)
    

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Lysine 78 mutated to Isoleucine; kinase dead

    Promoter

    CMV

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-GFPLaminA-R482W

    Plasmid

    #32709

    Depositor

    Wendy Bickmore

    Article

    Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

    Insert

    Lamin A (LMNA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R482W

    Promoter

    CMV

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP-RapR-FAK-YM-KD

    Plasmid

    #25929

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse, Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This pEGFP-RapR-FAK-YM-KD plasmid contains the FA…

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx-mCherry-LINXa3-nano

    Plasmid

    #73616

    Purpose

    Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3-nano
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDRf1-4CL5-AtSCT (JBEI-11557)

    Plasmid

    #87937

    Purpose

    Co-expression in S.cerevisiae of 4CL5 and AtSCT

    Depositor

    Dominique Loque

    Article

    Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

    Inserts

    AtSCT
    4CL5 (4CL5 Mustard Weed)
    

    Expression

    Yeast

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. DNM2 delta PRD

    Plasmid

    #168275

    Purpose

    Express PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.

    Depositor

    Cheng-han Yu

    Article

    Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

    Insert

    DNM2 (DNM2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa 1-742

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTXB1-TP4

    Plasmid

    #160087

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRE-mRuby3

    Plasmid

    #214923

    Purpose

    expression vector control - contitutive expression of mRuby33 alone

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    mRuby3
    

    Use

    Lentiviral

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PhyB-mCherry-CDC10

    Plasmid

    #51568

    Purpose

    Constitutively express bud neck localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    CDC10 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro

    Plasmid

    #44195

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    Cbx5 (Cbx5 Mouse)
    

    Use

    Lentiviral

    Tags

    Frb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…

    Mutation

    N-term chromo domain removed

    Promoter

    EF-1a

    Available Since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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