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pBEL2717

Plasmid

#201995

Purpose

Complementation of M. smegmatis MceG(E166Q)

Depositor

Gira Bhabha, Damian Ekiert

Article

Chen et al Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi

Insert

mceG

Expression

Bacterial

Mutation

Mutation E166Q

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2719

Plasmid

#201996

Purpose

Complementation of M. smegmatis MceG(Y178A)

Depositor

Gira Bhabha, Damian Ekiert

Article

Chen et al Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi

Insert

mceG

Expression

Bacterial

Mutation

Mutation Y178A

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dmchim4EBP_AB

Plasmid

#148374

Purpose

Insect Expression of Dmchim4EBP

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dmchim4EBP

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1840

Plasmid

#155150

Purpose

Expresses MlaF-6xHis

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-6xHis

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4E2H.210

Plasmid

#155212

Purpose

Expresses 4E2H.210 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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No promoter (ColE1)

Plasmid

#127951

Purpose

CFP fluorescent reporter with no promoter

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

No promoter

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKF293

Plasmid

#72857

Purpose

template for N-terminal 3x FLAG tag, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

3xFlag tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a.AQ_1575

Plasmid

#11416

Depositor

Sung-Hou Kim

Article

Shin et al Proc Natl Acad Sci U S A. 2002 Jun 11. 9

Insert

hypothetical (aq_1575 A. aeolicus)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
ED_p_044b

Plasmid

#246072

Purpose

ECOR31 mCpol D57A bacterial expression under T7 promoter, N-term His10-MBP-TEV

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Mutation

D57A

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PJ23102R1LuxR_PCTR1E2Crimson

Plasmid

#250892

Purpose

LuxR expressed under BBa_J23102; E2-Crimson under PCT synthetic promoter; component of BNeuAS6; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

E2-Crimson
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PCTR1EGFP_PJ23102R1LuxR

Plasmid

#250885

Purpose

EGFP expressed under PCT synthetic promoter and LuxR expressed under BBa_J23102 promoter; component of BNeuAS3; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

EGFP
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pART7

Plasmid

#247985

Purpose

Cloning vector to transiently express an inserted gene in plant cells under the control of the 35S CaMV promoter.

Depositor

Andrew Gleave, Christoph Plieth

Article

Gleave et al Plant Mol Biol. 1992 Dec;20(6):1203-7.

Type

Empty backbone

Expression

Plant

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1751

Plasmid

#246066

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pJex promoter; SC101 ori

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Panoptes locus

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1729

Plasmid

#246063

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Panoptes locus

Expression

Bacterial

Promoter

pTet promoter

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1866

Plasmid

#246069

Purpose

Bacterial expression of N4gp48 under control of the pJex promoter

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Anti CBASS 4

Expression

Bacterial

Promoter

pJex promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1398

Plasmid

#246059

Purpose

Bacterial expression of T4 Acb1 under control of the pJex promoter

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Anti CBASS 1

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1399

Plasmid

#246060

Purpose

Bacterial expression of T4 Acb2 under control of the pJex promoter

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Anti CBASS 2

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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PRC3MKO2

Plasmid

#193757

Purpose

Expresses mKO2 under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

mKO2

Expression

Bacterial

Promoter

PR

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AR

Plasmid

#183271

Purpose

All-in-One CRISPRko system with a guide RNA that targets AR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AR

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_C'4800EC

Plasmid

#176875

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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