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pSG5-FLAG-CaMKKalpha FL WT

Plasmid

#33327

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Tags

FLAG

Expression

Mammalian

Promoter

SV40

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
RCASBP-AP (E) (CC#39)

Plasmid

#15162

Depositor

Connie Cepko

Article

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-mScarletI

Plasmid

#179820

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_TALEN-FokI(WT)-BB_V3

Plasmid

#42588

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

TALEN-FokI(WT)-BB

Use

AAV; Adeno-associated virus ()

Tags

3XFlag

Expression

Mammalian

Promoter

CAG hybrid

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pL8_3xG34-DCAF8L2_X1

Plasmid

#191519

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG34-DCAF8L2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pL5_3xG22-DCAF8L2_X1

Plasmid

#191516

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG22-DCAF8L2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pT-35

Plasmid

#16092

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

SMT3 (SMT3 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
LDB107

Plasmid

#185423

Purpose

NUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporin

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

NUP1

Tags

HA

Expression

Yeast

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBT100.110

Plasmid

#122602

Purpose

clone CDA1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

CDA1

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_MCS_version2

Plasmid

#197950

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-AP-4 (Myc)3-σ4

Plasmid

#198331

Purpose

Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Tags

triple Myc tag

Expression

Mammalian

Mutation

silent substitution in codon 131

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLBC0030

Plasmid

#172777

Purpose

Level 0 module containing NT2_nYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS N-terminal eYFP (1-174)

Use

Synthetic Biology

Mutation

no

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0031

Plasmid

#172778

Purpose

Level 0 module containing CT_nYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS N-terminal eYFP (1-174)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0040

Plasmid

#172779

Purpose

Level 0 module containing NT2_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0041

Plasmid

#172780

Purpose

Level 0 module containing CT_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPD355 VPR-FRB (NLS)

Plasmid

#138847

Purpose

Constitutive expression of VPR-FRB under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKM498

Plasmid

#134215

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPT + CENPW

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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AP588-1

Plasmid

#71278

Purpose

Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagGFP

Tags

TEV::3xFlag and myc::TEV

Expression

Bacterial

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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