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pHH0103_LRP5_Ldl-recept-b

Plasmid

#109867

Purpose

Protein expression and purification of LRP5_Ldl-recept-b

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LRP5_Ldl-recept-b (LRP5 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_LRP5_Ldl-recept-b

Plasmid

#109785

Purpose

Protein expression and purification of LRP5_Ldl-recept-b

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LRP5_Ldl-recept-b (LRP5 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-α-tubulin

Plasmid

#136840

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-TUBA (DNMBP Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-α-tubulin

Plasmid

#136792

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-TUBA (DNMBP Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-3'short

Plasmid

#129749

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-5'short

Plasmid

#129748

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP

Plasmid

#129747

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 R312H

Plasmid

#124488

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed arginine 312 to histidine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236H

Plasmid

#124616

Purpose

Chimeric ETS domain: N236H

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (ETS1, SPI1 Human)

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a histidi…

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236Q

Plasmid

#124615

Purpose

Chimeric ETS domain: N236Q

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a glutami…

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236Y

Plasmid

#124614

Purpose

Chimeric ETS domain: N236Y

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a Tyrosine

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoBa

Plasmid

#121897

Purpose

Plasmid for making zebrafish apoBa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoBa (apoba Zebrafish)

Use

Unspecified

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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gh146

Plasmid

#106805

Purpose

expression of gRNA targeting FKTN

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FKTN (FKTN Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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gh186

Plasmid

#106847

Purpose

expression of gRNA targeting B3GNTL1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GNTL1 (B3GNTL1 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 G379R

Plasmid

#124489

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed glycine at 379 to arginine

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7

Plasmid

#118614

Purpose

expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

tetON CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1753_S2F-IMCg_doxy-CMV_mChe-Cdc42E7-E63'UTR

Plasmid

#118615

Purpose

expression of mChe-tagged cdc42E7-E6-3'UTR (swap) under doxy-inducible CMV promoter

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7-E6 3'UTR (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

tetON CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-PRM-3R

Plasmid

#112156

Purpose

Mammalian eGFP expression plasmid for PRM-3R.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-3R (ABL1 Human)

Expression

Mammalian

Mutation

contains only the PRM domain of ABl1

Promoter

CMV

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR160

Plasmid

#106054

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR160; Opto-GPR160; E8)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR160 (GPR160 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR83

Plasmid

#106035

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR83; Opto-GPR83; D1)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR83 (GPR83 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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