We narrowed to 7,648 results for: Lif;

1. pCS2-mCherry-Rab11a (S25N)

   Plasmid

   #184036

   Purpose

   Expresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

   Insert

   Rab11
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Serine 25 changed to Asparagine

   Promoter

   CMV IE94

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138129

   Purpose

   Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mClover3-anti-rabbit-IgG(Fc)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdestMB14_sur5p-dCas9-VP64

   Plasmid

   #177788

   Purpose

   Overexpression of dCas9-VP64 in C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   dCas9-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPSup_35Senh(fwd)_35Spr+synJ

   Plasmid

   #149422

   Purpose

   Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Insert

   35S core enhancer
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBITE-EGFRvIII-F263-OKT3

   Plasmid

   #190680

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-mCherry-Rab11a (Q70L)

   Plasmid

   #184037

   Purpose

   Expresses constitutively active mCherry Rab11a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

   Insert

   Rab11
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Glutamine 70 changed to Leucine

   Promoter

   CMV IE94

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SDHC-pHLuorin-sems

   Plasmid

   #107767

   Purpose

   labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

   Depositor

   Karin Busch

   Article

   Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

   Insert

   Subunit C of SDH fused to pHluorin (SDHC Human)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZS*11_4enh

   Plasmid

   #149423

   Purpose

   Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Inserts

   rbcS-E9 enhancer
   35S core enhancer
   Cab-1 enhancer
   AB80 enhancer
   

   Use

   Tags

   T120>A

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138133

    Purpose

    Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NET29A.Hs pEGFP-N2 (1181)

    Plasmid

    #62040

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET29A (TMEM120A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Splice variant

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPUR-mU6-sgRNA-2XPP7

    Plasmid

    #194504

    Purpose

    Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBITE-EGFRvIII-F271-OKT3

    Plasmid

    #190682

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. STOP-eGFP-ROSA26TV

    Plasmid

    #11739

    Depositor

    Klaus Rajewsky

    Article

    Sasaki et al Immunity. 2006 Jun . 24(6):729-39.

    Insert

    STOP-eGFP-ROSA26TV
    

    Use

    Cre/Lox; /stop

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKD184

    Plasmid

    #90953

    Purpose

    Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23114, PltetO-1, and PphsA

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXSE901BG

    Plasmid

    #91714

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL

    Plasmid

    #182012

    Purpose

    CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    CalR-HaloTag7-P30-SNAP-tag-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti6.3_CRKL-WT-V5

    Plasmid

    #196018

    Purpose

    Expresses CRKL WT, V5 tagged

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBSc + MShal

    Plasmid

    #16166

    Depositor

    Larry Salkoff

    Article

    Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

    Insert

    Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

20. p403-BrdU-Inc

    Plasmid

    #71789

    Purpose

    BrdU-incorporating plasmid with selectable marker HIS3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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