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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,173 results for: PEP

Showing: 4721 - 4740 of 5173 results

  1. C5A-DuET

    Plasmid
    #213192
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    C5A (C5AR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213192-plasmid-map-sequence-id-430757

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  2. CCKAR-DuET

    Plasmid
    #213195
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCKAR (CCKAR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213195-plasmid-map-sequence-id-430759

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  3. CCKBR-DuET

    Plasmid
    #213196
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCKBR (CCKBR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213196-plasmid-map-sequence-id-430760

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  4. AVPR1B-DuET

    Plasmid
    #213187
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AVPR1B (AVPR1B Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213187-plasmid-map-sequence-id-430752

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  5. AVPR2-DuET

    Plasmid
    #213188
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AVPR2 (AVPR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213188-plasmid-map-sequence-id-430753

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  6. ADRA2C-DuET

    Plasmid
    #213179
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADRA2C (ADRA2C Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213179-plasmid-map-sequence-id-430746

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  9. SpyTag003(V114T, V116T)-Talin rod-mCherry

    Plasmid
    #210027
    Purpose
    Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells
    Depositor
    Vesa Hytönen
    Article
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    SpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SpyTag003 V114T and V116T mutations to modify Spy…
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210027-plasmid-map-sequence-id-417751

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  11. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G

    Plasmid
    #194462
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-JAG1 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194462-plasmid-map-sequence-id-384265

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  12. pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G

    Plasmid
    #194460
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-JAG1 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194460-plasmid-map-sequence-id-384267

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  16. pS238D1::sptse5-CT_phoA-lacZalpha

    Plasmid
    #192961
    Purpose
    Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT_phoA-lacZ_
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1317) fused to phoA-lacZal…
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192961-plasmid-map-sequence-id-380611

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  18. pStr-KDEL_EPV-CXCL9-mCherry-SBP

    Plasmid
    #170678
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    CXCL9 (CXCL9 Human, Synthetic)
    streptavidin-KDEL
    Use
    Tags
    SBP and mCherry
    Expression
    Mammalian
    Mutation
    Changed threonine 23 to glutamate (T23E)
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170678-plasmid-map-sequence-id-335801

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  19. pDONR207-MUM2sp-Citrine-ADPG2

    Plasmid
    #170725
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    Use
    Gateway donor vector / entry clone
    Tags
    Citrine tag (714bp)
    Expression
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    170725-plasmid-map-sequence-id-345738

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  20. pDONR207-MUM2sp-ADPG2

    Plasmid
    #170723
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170723-plasmid-map-sequence-id-345619

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Showing: 4721 - 4740 of 5173 results