We narrowed to 11,287 results for: aga

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KTK_039

Plasmid

#180561

Purpose

Entry vector containing L3S2P21 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P21 terminator

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_038

Plasmid

#180560

Purpose

Entry vector containing L3S2P11 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P11 terminator

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

Plasmid

#168252

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

Plasmid

#168253

Purpose

"label stable F-actin; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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L2_NOP1gRNA-Cas9-CsA

Plasmid

#136139

Purpose

Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0065

Plasmid

#177019

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

1-deoxy-D-xylulose 5-phosphate synthase

Use

Synthetic Biology

Mutation

transit peptide removed, amplified from cDNA, con…

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSC51

Plasmid

#104825

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.12g075700, Glyma.11g145900

Use

CRISPR

Expression

Plant

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-1

Plasmid

#159931

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-2

Plasmid

#159932

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ncapd3-L

Plasmid

#122329

Purpose

Expresses sgRNA targeting mouse Ncapd3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ncapd3

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pZ_P-ICL1-LacI-Mit1AD

Plasmid

#126721

Purpose

LacI-Mit1AD on pPICZ B expression vector

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Mit1 activation domain coding sequence

Expression

Yeast

Promoter

ICL1

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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gh169

Plasmid

#106831

Purpose

expression of gRNA targeting ABO

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ABO (ABO Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 P294A

Plasmid

#112102

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

P294A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-sgTAF12(human)#4.5

Plasmid

#105987

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human TAF12-HFD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-neo-sgTAF12(mouse)#4.1

Plasmid

#105588

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting TAF12 HFD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-puro-N3FLAG-MYB-del(TAD)

Plasmid

#105597

Purpose

retrovirally express mouse MYB-del(TAD) with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB without TAD domain (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

deletion of TAD (AA 275-327)

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-TAF4(827-909)

Plasmid

#105616

Purpose

retrovirally express express mouse TAF4 HFD with GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD-827-909 (Taf4 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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SP65U1-135a-1

Plasmid

#105819

Purpose

plasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmid

Depositor

Cecilia Mannironi

Article

Mannironi et al PLoS One. 2013 Sep 4;8(9):e73385. doi: 1

Insert

MIRNA135A1 (Mir135a-1 Mouse)

Expression

Mammalian

Promoter

U1

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-zfh1_spikeIn-1

Plasmid

#86387

Purpose

Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter

Use

Stap-seq spike in vector

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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NUDC D10.4 gRNA

Plasmid

#90807

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUDC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NUDC (Guide Designation D10.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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