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KCC2-pHext

Plasmid

#104076

Purpose

To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

Depositor

Igor Medyna

Article

Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Tags

pHluorine tag inserted in the second putative tra…

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

UbC

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

Plasmid

#169469

Purpose

Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV NucleoBAS N354K-I372V

Plasmid

#63574

Purpose

Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

NucleoBAS N354K-I372V

Tags

SV40 NLS

Expression

Mammalian

Mutation

N345K and I372V

Promoter

CMV

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only
pMEL15

Plasmid

#107921

Purpose

nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pROS12

Plasmid

#107926

Purpose

hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pROS15

Plasmid

#107929

Purpose

nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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phBG-P53DD

Plasmid

#149707

Purpose

In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

Depositor

Erika Sasaki

Article

Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

Insert

Trp53 (Trp53 Mouse)

Use

Rna transcription

Mutation

deleted amino acid 14-301

Promoter

T7

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pROS14

Plasmid

#107928

Purpose

LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pROS13

Plasmid

#107927

Purpose

kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMEL14

Plasmid

#107920

Purpose

LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPHR-iRFP713-NLS-VP16

Plasmid

#103823

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-iRFP713-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-NLS-VP16

Plasmid

#103821

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-mCherry-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

Plasmid

#169467

Purpose

Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMEL11

Plasmid

#107917

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-NLS-VP16

Plasmid

#103822

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD(QQQ)-E12

Plasmid

#26003

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Mutation

Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pscAAV_hSyn-TetOn3G

Plasmid

#254465

Purpose

Self-complementary AAV transfer vector with oversized payload comprising neuron-specific hSyn1 promoter-driven Tet-On 3G and mScarlet-I3. For co-transduction with pAAV_TRE3G-NTVE(PABP).

Depositor

Gil Westmeyer

Article

Armbrust et al Nat Commun. 2026 Apr 25;17(1):3812. doi:

Insert

Tet-On3G(V9I)_P2A_NLS-mScalet-I3

Use

AAV

Promoter

hSyn

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pPiggyBac_NTVE(PABP)-dVSVG-HA

Plasmid

#254462

Purpose

Transposon encoding doxycycline-inducible NTVE(PABP) with HA tag. Co-expression of PiggyBac transposase required. Tet-On 3G and puromycin N-acetyltransferase are co-expressed via CAG promoter.

Depositor

Gil Westmeyer

Article

Armbrust et al Nat Commun. 2026 Apr 25;17(1):3812. doi:

Insert

NTVE(PABP)_P2A_dVSV-G-HA

Expression

Mammalian

Promoter

TRE3G

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pPiggyBac_NTVE(PABP)-dVSVG-FLAG

Plasmid

#254461

Purpose

Transposon encoding doxycycline-inducible NTVE(PABP) with FLAG tag. Co-expression of PiggyBac transposase required. Tet-On 3G and puromycin N-acetyltransferase are co-expressed via CAG promoter.

Depositor

Gil Westmeyer

Article

Armbrust et al Nat Commun. 2026 Apr 25;17(1):3812. doi:

Insert

NTVE(PABP)_P2A_dVSV-G-FLAG

Expression

Mammalian

Promoter

TRE3G

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pPD231 PPH-T2A-dCas9-NFZ-P2A-BFP

Plasmid

#249155

Purpose

Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

PPH-T2A-dCas9-NFZ-P2A-mTagBFP2

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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