We narrowed to 15,789 results for: LEA

1. pCAGGS-N-TEV-Twin-Strep

   Plasmid

   #202526

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site and Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ADAM10-HA

   Plasmid

   #65106

   Purpose

   Expression of HA tagged ADAM10 in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   ADAM10 (ADAM10 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ADAM17-HA

   Plasmid

   #65105

   Purpose

   Expression of HA tagged ADAM17 (TACE) in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   ADAM17 (ADAM17 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3_LuxSit_i_mTagBFP2

   Plasmid

   #194300

   Purpose

   Mammalian expression of the de novo luciferase, LuxSit-i

   Depositor

   David Baker, Andy Yeh

   Article

   Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

   Insert

   LuxSit-i
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57-MCS1-P2A-MCS2 (Hygro)

   Plasmid

   #80922

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57-GFP-2A-MCS

   Plasmid

   #71783

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

   Plasmid

   #86420

   Purpose

   N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   CDC42pr-GFP1-10
   

   Use

   Tags

   GFP1-10

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-ER alpha

   Plasmid

   #28230

   Purpose

   Mammalian expression of Estrogen Receptor alpha fused to EGFP

   Depositor

   Michael Mancini

   Article

   Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

   Insert

   Estrogen Receptor alpha (ESR1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX Nup62Fv2GFP

   Plasmid

   #139683

   Purpose

   Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

   Depositor

   Edward Campbell

   Article

   Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

   Insert

   Nup62 (NUP62 Human)
   

   Use

   Lentiviral

   Tags

   2 copies of GFP and Dimerization domain FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE2-Bla(HA–RILP-FLAG)

    Plasmid

    #102424

    Purpose

    Dual tagged RILP for expression in mammalian cells.

    Depositor

    Steven Weinman

    Article

    Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    Rab interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-Responsive

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-HA-Ubiquitin-K27

    Plasmid

    #22902

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K27 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-MCS1-P2A-MCS2 (Blast)

    Plasmid

    #80921

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaM-6

    Plasmid

    #172771

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-6
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCL1313

    Plasmid

    #217955

    Purpose

    Expression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-C-TEV-Twin-Strep

    Plasmid

    #202527

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-Foxo1

    Plasmid

    #17551

    Depositor

    Domenico Accili

    Article

    Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

    Insert

    pEGFP-N1 Foxo1 (Foxo1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTRIPZ shNS

    Plasmid

    #127696

    Purpose

    Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shNS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Perturb-FISH

    Plasmid

    #220626

    Purpose

    expression of gRNAs under U6T7 promoter

    Depositor

    Samouil Farhi

    Article

    Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    BsmBI

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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