We narrowed to 15,991 results for: LEA

1. ER-GCaMP6-150

   Plasmid

   #86918

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-150
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRY2olig-GP

   Plasmid

   #218918

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-GP
   

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TR-Csy4-R115A/R119A

   Plasmid

   #80696

   Purpose

   Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   Csy4-R115A/R119A
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Arg115 and Arg119 both mutated to Ala

   Promoter

   CBA

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER-GCaMP6-210

   Plasmid

   #86919

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-210
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. UBXD1-GFP

   Plasmid

   #86464

   Purpose

   Expression of human UBXD1 with C-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   UBXD1 (UBXN6 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1_VNp6-mNeongreen

   Plasmid

   #182388

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-mNeongreen-His
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-LahT150

   Plasmid

   #166785

   Purpose

   Cleavage of double glycine motif in peptides to remove the leader sequence

   Depositor

   Wilfred van der Donk

   Article

   Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

   Insert

   LahT150
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX Nup62Fv2GFP

   Plasmid

   #139683

   Purpose

   Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

   Depositor

   Edward Campbell

   Article

   Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

   Insert

   Nup62 (NUP62 Human)
   

   Use

   Lentiviral

   Tags

   2 copies of GFP and Dimerization domain FKBP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV sgRNA Expression Plasmid

   Plasmid

   #174540

   Purpose

   Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

   Depositor

   Ormond MacDougald

   Article

   Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Tags

   None

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CuO-LacI-NLS–SNAPf

    Plasmid

    #227368

    Purpose

    Lentiviral expression of LacI–SNAP

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    LacI NLS SNAPf
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-TORCAR-NES

    Plasmid

    #175015

    Purpose

    Cytosol-targeted TORCAR.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    TORCAR-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. IK349

    Plasmid

    #233655

    Purpose

    Dox-inducible red light-inducible Flp-C; Flp-397C-PhyA

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Flp-397C-PhyA
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    TRE3G

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C3-ZO-2

    Plasmid

    #27422

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pTRE-TIGHT-EGFP-donor fw copy

    Plasmid

    #22074

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    TETO-eGFP
    

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. PS Intein/C1A Bid

    Plasmid

    #242025

    Purpose

    PS Intein Bid construct containing the C1A mutation in gp41-1, which abolishes its autocatalytic splicing activity.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein/C1A Bid
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. IK331

    Plasmid

    #233653

    Purpose

    Dox-inducible blue light-inducible Cre-N; Cre-N229-pmag

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Cre-N229-pmag
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. IK332

    Plasmid

    #233654

    Purpose

    Dox-inducible blue light-inducible Cre-C; Cre-230C-nmag

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Cre-230C-nmag
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-ADAM10-HA

    Plasmid

    #65106

    Purpose

    Expression of HA tagged ADAM10 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM10 (ADAM10 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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