We narrowed to 12,019 results for: pcdna

1. pcDNA3.1-kappa-HA-dL5-BKa

   Plasmid

   #73212

   Purpose

   Expresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Pratt et al Bioconjug Chem. 2015 Sep 16;26(9):1963-7

   Insert

   BKalpha (Kcnma1 Mouse)
   

   Use

   Tags

   dL5**

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cry2 mCherry GRK3 ct in pcDNA3.1

   Plasmid

   #64213

   Purpose

   mCherry fused between Cry2 and GRK3 ct to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 PHR (CRY2 Mustard Weed)
   GRK3 ct (GRK3 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5/FRT/TO HIS DNAJB9

   Plasmid

   #19559

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PKCsub-YPet-NES

   Plasmid

   #138207

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKCsub-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-optoB2-LYY-YFP

   Plasmid

   #75271

   Purpose

   G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   G-protein independent version of optoB2
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   point mutations at L72F,Y136G,Y224A

   Promoter

   Available Since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Yellow Cameleon-Nano30/pcDNA3

   Plasmid

   #51963

   Purpose

   mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano30
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-HCMVgH-8his (TB40/E)

   Plasmid

   #136445

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Short GGSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular domain (a.a. 1-717). Codon-optimize…

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

   Plasmid

   #112829

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

   Insert

   LL5BIP (2410002F23Rik Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HtrA2-EGFP S306A

   Plasmid

   #14122

   Depositor

   L. Miguel Martins

   Article

   Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S306A

   Promoter

   Available Since

   March 7, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1 Myc-huMEKK3 (K391A)

    Plasmid

    #44157

    Depositor

    Xin Lin

    Article

    Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

    Insert

    MEKK3 (K391A) (MAP3K3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K391A, kinase-dead mutant

    Promoter

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-HA-REDD1 48-232 (#182)

    Plasmid

    #99920

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 48-232

    Promoter

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO GFP HSPA14

    Plasmid

    #19488

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA14 (HSPA14 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-GFP-EBA175-Flag

    Plasmid

    #154970

    Purpose

    Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    GFP and signal peptide (residues 1 to 32) from TG…

    Expression

    Mammalian

    Mutation

    insert codes for EBA175 residues 1284 to 1462

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 FRT/TO MycLAP-STIL 5A

    Plasmid

    #80267

    Purpose

    mammalian expression plasmid for MycLAP-STIL 5A

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    S1103A, S1108A, S1111A, S1116A, T1119A

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 c-SRC (250-536)

    Plasmid

    #42208

    Depositor

    Robert Lefkowitz

    Article

    Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

    Insert

    c-SRC (250-536) (SRC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 250-536

    Promoter

    CMV

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-sACE2-T92Q-Fc(IgG1)

    Plasmid

    #145170

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

    Plasmid

    #192445

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-mCherry-TSC2-NLS

    Plasmid

    #192441

    Purpose

    Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2-NLS (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid

    #192443

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3 Flag MKK7a1 (ala)

    Plasmid

    #14539

    Depositor

    Roger Davis

    Article

    Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

    Insert

    MKK7a1(Ala) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-198Ala Thr-202Ala

    Promoter

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only