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p361_DG

Plasmid

#234683

Purpose

Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Kpn1

Plasmid

#203302

Purpose

For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Publication

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-EcoR1

Plasmid

#203305

Purpose

For expression of eukaryotic genes of interest with CMV promoter with EcoRI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Publication

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PB_TRE_AP10-PDGFR_EF1a_mOrange

Plasmid

#186308

Purpose

Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Publication

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

AP10-PDGFR
mOrange

Tags

HA, myc

Expression

Mammalian

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pFRTpsiCHECK_SEAP

Plasmid

#154461

Purpose

Expression of SEAP and firefly luciferase

Depositor

Markus Landthaler

Publication

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SEAP

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA4

Plasmid

#174034

Purpose

In vitro transcription of anti-CRISPR AcrIIA4 mRNA

Depositor

James Gagnon

Publication

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA4

Use

In vitro transcription

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA2

Plasmid

#174033

Purpose

In vitro transcription of anti-CRISPR AcrIIA2 mRNA

Depositor

James Gagnon

Publication

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA2

Use

In vitro transcription

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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RGG-NanoLuc-RGG

Plasmid

#176642

Purpose

NanoLuc enzyme fused to LAF-1 RGG domain

Depositor

Daniel Hammer

Publication

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

RGG-NanoLuc-RGG

Expression

Bacterial

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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C121-E12: NanoLuc

Plasmid

#162891

Purpose

Standard Gateway entry clone encoding NanoLuc luciferase

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pKP660

Plasmid

#160812

Purpose

Expression of ExbB, ExbD proteins

Depositor

Kathleen Postle

Publication

Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

Insert

exbB, exbD

Expression

Bacterial

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-G2

Plasmid

#137091

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Publication

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GG

Use

Synthetic Biology

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBTK620

Plasmid

#110620

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Jan. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK621

Plasmid

#110621

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-clbB

Plasmid

#114155

Purpose

Expresses full-length ClbB

Depositor

Emily Balskus

Publication

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbB

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK550d

Plasmid

#110617

Purpose

BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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YCe1731 HC_Kan_RFP-p24

Plasmid

#100634

Purpose

EMMA toolkit part-entry vector position 24

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT31

Plasmid

#89666

Purpose

expression of doubleTAL staple protein dT31 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Publication

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT31

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT27

Plasmid

#89665

Purpose

expression of doubleTAL staple protein dT27 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Publication

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT27

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-alpha1-chimaerin

Plasmid

#59317

Purpose

Expression plasmid for GFP-tagged alpha1-chimaerin (GFP at N-terminus of alpha1-chimaerin)

Depositor

Peter Scheiffele

Publication

Beg et al Neuron. 2007 Sep 6;55(5):768-78.

Insert

alpha1-chimaerin

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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LIIIalpha fin

Plasmid

#54381

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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