We narrowed to 727 results for: ELP

1. pAAVtri-CMV-eGFP

   Plasmid

   #230932

   Purpose

   Used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.

   Depositor

   Qizhao Wang

   Article

   Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-HA_N-human Prkd2 without kinase domain

   Plasmid

   #138411

   Purpose

   Express Prkd2 without kinase domain in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

   Insert

   Prkd2 without kinase domain (PRKD2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   kinase domain deleted from human Prkd2

   Promoter

   CMV

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-HA_N-mouse Bcl6

   Plasmid

   #138410

   Purpose

   Express mouse Bcl6 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

   Insert

   B cell lymphoma 6 (Bcl6 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Lrrc40-trunc-IRES-BFP

   Plasmid

   #117268

   Purpose

   Retroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFP

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Lrrc40 (Lrrc40 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The insert is truncated - deletion of bp 334-407 …

   Promoter

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEX-P301L Tau

   Plasmid

   #176267

   Purpose

   This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

   Depositor

   Tsuneya Ikezu

   Article

   You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

   Has Service

   AAV1

   Insert

   Flex-P301L tau (MAPT )
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCag FlpO-2A-Cre EV

   Plasmid

   #129419

   Purpose

   episomal expression of FlpO and Cre recombinases

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/Chick β-actin (CAG)

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCag SE (Self-excising) FlpO-2A-Cre EV

   Plasmid

   #130986

   Purpose

   episomal expression of FlpO and Cre recombinases, self excising

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   protamine intron added to FlpO to prevent bacteri…

   Promoter

   CMV/Chick β-actin (CAG)

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

   Plasmid

   #129421

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-3Flag
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   3X Flag epitope tag

   Expression

   Mutation

   Promoter

   none (3X polyA to mitigate episomal expression)

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MADR pDonor smFP-HA (dark) WPRE

   Plasmid

   #131007

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-HA WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   HA - 10 total HA epitope tages

   Expression

   Mammalian

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid

    #131006

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    V5 - 10 total of V5 epitope tag

    Expression

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MADR pDonor smFP-Myc (dark) WPRE

    Plasmid

    #130987

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-Myc WPRE
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    Myc - 10 total Myc tags

    Expression

    Mutation

    "dark" variant with GGG fluorophore ver…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MADR pDonor smFP-FLAG (dark) WPRE

    Plasmid

    #131005

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-FLAG WPRE
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    FLAG - 10 total FLAG tags

    Expression

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131462

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    H3F3A K27M, Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131461

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131463

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    H3F3A G34R, Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

    Plasmid

    #129420

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    TagBFP2-V5 tag

    Expression

    Mutation

    G12V

    Promoter

    none (3x polyA to mitigate episomal expression)

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid

    #131730

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    TagBFP2-V5 tag

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL4723-P1

    Plasmid

    #86173

    Purpose

    LevelP cloning Vector

    Depositor

    Mark Youles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAdDeltaF6

    Plasmid

    #112867

    Purpose

    AAV helper plasmid

    Depositor

    James M. Wilson

    Insert

    E4, E2a and VA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOpen-RNAse H

    Plasmid

    #165575

    Purpose

    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    RNAse H
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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