Addgene

We narrowed to 547 results for: PMCH

Showing: 461 - 480 of 547 results

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PDZb-beta2(616-951)-mCherry

Plasmid

#100736

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

PDZb and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2–mPKCε-CAT-HA

Plasmid

#190483

Purpose

Expresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAX

Depositor

Diomedes Logothetis

Article

Gada et al J Biol Chem. 2022 May;298(5):101893. doi

Insert

mCherry-CRY2–mPKCε-CAT-HA (Prkce Mouse, Synthetic)

Use

Tags

Fused to CRY2, HA, and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn-iTrkA-mCh-CRY2

Plasmid

#106169

Purpose

mammalian expression of Lyn-tagged intracellular domain of TrkA (aa 450-799) fused to mCherry and CRY2PHR (aa 1-498)

Depositor

Bianxiao Cui

Article

Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

Insert

Lyn iTrkA mCherry CRY2PHR (CRY2 Mustard Weed, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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CRY-tTA (B702)

Plasmid

#92032

Purpose

Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

tTA2 (TetR fused to VP64)

Expression

Mammalian

Mutation

NLS on CRY2 is mutated

Promoter

CMV

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-TPD53

Plasmid

#172457

Purpose

Expression of Tumor Protein D52 like 1 (TPD53/TPD52L1) with N-terminal mCherry-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD53 (TPD52L1 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PHB2 S39A-mCherry

Plasmid

#232786

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2 non-phosphorylatable on Ser39

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed Ser 39 to Ala

Promoter

CMV

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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PHB2 S39D-mCherry

Plasmid

#232787

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed Serine 39 to Aspartate

Promoter

CMV

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-FKBPflex-ELL-CAAX

Plasmid

#220076

Purpose

an extra long linker attached to a CAAX domain with an mCherry tag

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

CAAX (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2C

Plasmid

#202736

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2C (PIP4K2C, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2CD280K

Plasmid

#202737

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2C (D278K) (PIP4K2C, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2BD278K

Plasmid

#202735

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A (3-108):[GGSA]4GG:PIP4K2B (D280K) (PIP4K2B, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2A

Plasmid

#202732

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (PIP4K2A, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2AD273K

Plasmid

#202733

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (D266K) (PIP4K2A, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-STAT2

Plasmid

#183931

Purpose

Optogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-STAT2 (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-Rta

Plasmid

#183930

Purpose

Optogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-Rta (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-VPR

Plasmid

#183928

Purpose

Optogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VPR (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-FUSN

Plasmid

#183932

Purpose

Optogenetic PHR domain coupled to GFP and FUSN domain with a high phase separation propensity; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-FUSN (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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CRY-GalVP16 (B695)

Plasmid

#92031

Purpose

Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

GalBD-VP16AD

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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mcherry-DN KASH

Plasmid

#125553

Purpose

mcherry labeled DN-KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

CMV

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-mCh

Plasmid

#120169

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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