We narrowed to 21,238 results for: grna

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pCRISPR::rpsL

Plasmid

#44505

Purpose

A crRNA expression plasmid specific to the rpsL allele.

Depositor

Luciano Marraffini

Publication

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR::rpsL (rpsL )

Use

CRISPR; E.coli

Available since

April 16, 2013

Availability

Academic Institutions and Nonprofits only

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pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

Plasmid

#64216

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP

Expression

Mammalian

Promoter

CBh and U6

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone

Pooled library

#1000000075

Purpose

Genome-wide CRISPR gRNA pooled library for activation of mouse genes

Depositor

Feng Zhang

Available since

March 17, 2016

Availability

Academic Institutions and Nonprofits only
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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AAV-U6gRNA1-U6gRNA2-TnT-Cre

Plasmid

#87682

Purpose

AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

Depositor

William Pu

Publication

Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

Inserts

gRNA1
gRNA2
Cre

Use

AAV

Promoter

U6 and cTnT

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pC0052-REPAIR non-targeting guide clone into pC0043

Plasmid

#103868

Purpose

Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

non-targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRGEB32

Plasmid

#63142

Purpose

Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

Depositor

Yinong Yang

Publication

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Rice snoRNA U3 promoter for gRNA/PTG expression a…

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Publication

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pU6-tmpknot-GG-acceptor

Plasmid

#174039

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Publication

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pFREE_Cm

Plasmid

#92052

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Publication

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCE27

Plasmid

#174405

Purpose

C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

Depositor

Clarissa Nobile

Publication

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2

Use

CRISPR

Expression

Bacterial

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Human SAM library in lenti sgRNA(MS2)_puro backbone

Pooled library

#1000000074

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

Feng Zhang

Available since

March 17, 2016

Availability

Academic Institutions and Nonprofits only
pFREE_Amp

Plasmid

#92051

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Publication

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG

Plasmid

#70183

Purpose

Inducible expression of guide RNA with fluorescent GFP reporter

Depositor

Marco Herold

Publication

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

H1-Tet-sgrna cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

80 Articles

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pCRCT

Plasmid

#60621

Purpose

Plasmid encoding iCas9, tracrRNA and crRNAs

Depositor

Huimin Zhao

Publication

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Publication

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

cas9

Use

CRISPR; Lambda red recombinase

Expression

Bacterial

Promoter

native cas9 promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

67 Articles

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pJW1219

Plasmid

#61250

Purpose

CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E)

Use

CRISPR

Expression

Worm

Promoter

U6 promoter

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBS U6a gRNA

Plasmid

#158961

Purpose

gRNA backbone driven by zebrafish U6a promoter

Depositor

Michael Brand

Publication

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

zf U6a promoter
gRNA backbone

Use

CRISPR

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Publication

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

192 Articles

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