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Plasmid#106426PurposeH-RAN-RFP6 (RANbody RFP6::Sm-HAtag)DepositorInsertH-RAN-RFP6
UseTagsHA, His, and Multiple HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-H-RAN-H2A2B
Plasmid#106419PurposeH-RAN-H2A2B (RANbody H2A2B::Sm-HAtag)DepositorInsertH-RAN-H2A2B
UseTagsHA, His, and Multiple HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M-RAN-RFP6
Plasmid#106428PurposeM-RAN-RFP6 (RANbody RFP6::Sm-MYCtag)DepositorInsertM-RAN-RFP6
UseTagsHA, His, and Multiple MYCExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-mTagBFP2
Plasmid#220747PurposemTagBFP2 expression in mammalian cells. Reporter plasmid.DepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationPromoter12xUAS with TATA-box minimal promoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-nls
Plasmid#108882PurposeMultisite gateway vector for 5' tagging with an N-terminal nuclear localisation sequenceDepositorInsertnls
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceOct. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M-RAN-H2A2B
Plasmid#106420PurposeM-RAN-H2A2B (RANbody H2A2B::Sm-MYCtag)DepositorInsertM-RAN-H2A2B
UseTagsHA, His, and Multiple MYCExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Y-RAN-H2A2B
Plasmid#106421PurposeY-RAN-H2A2B (RANbody H2A2B::Chicken IgY)DepositorInsertY-RAN-H2A2B
UseTagsHA and HisExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
WIW_Scaffold2
Plasmid#205574PurposeNanobody that inhibits seeding of tau fibrilsDepositorInsertWIW_Scaffold2
UseTags6X HisExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1 GBP (GBP-mCherry)
Plasmid#162879PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with mCherryDepositorInsertGFP Binding Protein
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-Nb127D01-hIgG
Plasmid#171564PurposeExpresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
UseTagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT2-LaG16-3W
Plasmid#234425PurposeExpress APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linkerDepositorInsertAPT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT1-LaG16-3W
Plasmid#234424PurposeExpress APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linkerDepositorInsertAPT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_H6_His
Plasmid#201719PurposeExpression of nanobodyDepositorInsertVHH_H6 (S )
UseTagsHexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sybody Generation Toolbox
Plasmid Kit#1000000160PurposeThe Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.DepositorAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
UseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
UseTags3XFLAG and 6XHisExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-miRFP670nano3
Plasmid#184664PurposeMonomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmidDepositorInsertmiRFP670nano3
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDG02583
Plasmid#104129PurposeOptimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)DepositorInsertNEDD8-specific protease 1 (NEDP1)
UseTags14x Histidine tag, Maltose binding protein, and S…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBMNb-cALFA
Plasmid#159991PurposeFor the protein expression with ALFA tag at C-terminus in mammalian cellsDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationCMV promoter, WPREPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV_LaG17_synNotch_TetRVP64
Plasmid#79128PurposeLentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)DepositorInsertLaG17 GFP nanobody synNotch TetRVP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only