We narrowed to 6,134 results for: nls

1. pCS-nlstdTomato

   Plasmid

   #132985

   Purpose

   full length of nuclear localized tdTomato in pCS expression vector

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Insert

   tdTomato
   

   Use

   Tags

   NLS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-2a-6MTnlstdTomato

   Plasmid

   #67699

   Purpose

   3' entry clone with 6xmyc nuclear localized tdTomato

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   tdTomato
   

   Use

   Tags

   2A-6xMyc-NLS

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. R26/P-nlsrtTA

   Plasmid

   #46183

   Depositor

   Rudolf Jaenisch

   Article

   Wutz et al Nat Genet. 2002 Feb;30(2):167-74. Epub 2

   Inserts

   nlsrtTA
   pgk-puro
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

4. LN-entry+NLS

   Plasmid

   #128011

   Purpose

   Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag-SV40NLS
   

   Use

   Lamdan entry vector with nls to generate tetherin…

   Tags

   LN-3xFlag-SV40NLS

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-GAI(1-92)

   Plasmid

   #37317

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   contains aa 1-92

   Promoter

   CMV

   Available since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

6. M_NLS 67kDa

   Plasmid

   #201351

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   (NLS)SV40A5-EGFP-6PrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NLS-mRFP-V1

   Plasmid

   #124266

   Purpose

   Nuclear localised mRFP reporter construct with one half of split Venus

   Depositor

   Eric Schirmer

   Insert

   mRFP
   

   Use

   Tags

   NLS and Venus1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3E-CMVmin:nlsGFP pA

   Plasmid

   #80821

   Purpose

   CMVmin-promoted nlsGFP with pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-CMV-nlsGFP pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVmin

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNLS-LSSmKate1-N1

   Plasmid

   #31870

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Promoter

   CMV

   Available since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-21a_2xNLS_FnCpf1_MBP_protein_expression

    Plasmid

    #120801

    Purpose

    Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    2xNLS_FnCpf1
    

    Use

    CRISPR

    Tags

    MBP and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Use

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNLS-LSSmKate2-N1

    Plasmid

    #31871

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    Available since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-BE3-2NLS

    Plasmid

    #119031

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT2iC6Dronpa2nlsER4(#636)

    Plasmid

    #184067

    Purpose

    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    Dronpa2-2nls-ERT2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. NLS-mRFP-V2

    Plasmid

    #124267

    Purpose

    Nuclear localised mRFP reporter construct with one half of split Venus

    Depositor

    Eric Schirmer

    Insert

    mRFP
    

    Use

    Tags

    NLS and Venus2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME-6MTnlstdTomato2A

    Plasmid

    #67685

    Purpose

    Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    tdTomato
    

    Use

    Tags

    2A and 6xMyc-NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-BE3-3NLS

    Plasmid

    #119032

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-P4:gs:nls:FKBP

    Plasmid

    #135984

    Purpose

    Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    P4 coiled-coil fused to the FKBP domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-His:FRB:nls:VPR

    Plasmid

    #135987

    Purpose

    Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    FRB domain fused to the VPR activation domain
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LI B-C N-NLS KOZ c2

    Plasmid

    #54443

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    Nuclear localization signal (NLS) of SV40 large T antigen
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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