We narrowed to 6,241 results for: nls

1. p16 nlsGPS1-NR

   Plasmid

   #101082

   Purpose

   GA biosensor expression plasmid

   Depositor

   Wolf Frommer, Alexander Jones

   Article

   Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

   Insert

   nlsGPS1-NR
   

   Tags

   cMyc

   Expression

   Plant

   Mutation

   AtGAI E51K E54R, AtGID1C S114A F115A

   Promoter

   p16 (AT3G60245)

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

   Plasmid

   #120506

   Purpose

   Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC H84NLS (MYC Human)
   

   Use

   Lentiviral

   Mutation

   Deletion of nuclear localization signal sequence

   Promoter

   EF1a

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVMagFRET-6-NLS

   Plasmid

   #50757

   Purpose

   Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

   Depositor

   Maarten Merkx

   Article

   Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

   Insert

   gene encoding for MagFRET-6 with NLS
   

   Expression

   Mammalian

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS-nlstdTomato

   Plasmid

   #132985

   Purpose

   full length of nuclear localized tdTomato in pCS expression vector

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Insert

   tdTomato
   

   Tags

   NLS and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1xNLS-pMJ915v2

   Plasmid

   #88915

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-2a-6MTnlstdTomato

   Plasmid

   #67699

   Purpose

   3' entry clone with 6xmyc nuclear localized tdTomato

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   tdTomato
   

   Tags

   2A-6xMyc-NLS

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. R26/P-nlsrtTA

   Plasmid

   #46183

   Depositor

   Rudolf Jaenisch

   Article

   Wutz et al Nat Genet. 2002 Feb;30(2):167-74. Epub 2

   Inserts

   nlsrtTA
   pgk-puro
   

   Use

   Mouse Targeting

   Available Since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

8. NLS-GAI(1-92)

   Plasmid

   #37317

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   contains aa 1-92

   Promoter

   CMV

   Available Since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

9. LN-entry+NLS

   Plasmid

   #128011

   Purpose

   Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag-SV40NLS
   

   Use

   Lamdan entry vector with nls to generate tetherin…

   Tags

   LN-3xFlag-SV40NLS

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-mRFP-V1

    Plasmid

    #124266

    Purpose

    Nuclear localised mRFP reporter construct with one half of split Venus

    Depositor

    Eric Schirmer

    Insert

    mRFP
    

    Tags

    NLS and Venus1

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-CMVmin:nlsGFP pA

    Plasmid

    #80821

    Purpose

    CMVmin-promoted nlsGFP with pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-CMV-nlsGFP pA
    

    Expression

    Mammalian

    Promoter

    CMVmin

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. M_NLS 67kDa

    Plasmid

    #201351

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A5-EGFP-6PrA
    

    Expression

    Mammalian

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNLS-LSSmKate1-N1

    Plasmid

    #31870

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS
    

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    CMV

    Available Since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-21a_2xNLS_FnCpf1_MBP_protein_expression

    Plasmid

    #120801

    Purpose

    Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    2xNLS_FnCpf1
    

    Use

    CRISPR

    Tags

    MBP and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNLS-LSSmKate2-N1

    Plasmid

    #31871

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS
    

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Available Since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-BE3-2NLS

    Plasmid

    #119031

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2iC6Dronpa2nlsER4(#636)

    Plasmid

    #184067

    Purpose

    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    Dronpa2-2nls-ERT2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. NLS-mRFP-V2

    Plasmid

    #124267

    Purpose

    Nuclear localised mRFP reporter construct with one half of split Venus

    Depositor

    Eric Schirmer

    Insert

    mRFP
    

    Tags

    NLS and Venus2

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-6MTnlstdTomato2A

    Plasmid

    #67685

    Purpose

    Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    tdTomato
    

    Tags

    2A and 6xMyc-NLS

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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