We narrowed to 63,817 results for: cell

1. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-mCherry-Otx2shRNA1

   Plasmid

   #73979

   Purpose

   Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse Otx2 gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTrcHisA-rat-PTP1B

   Plasmid

   #35987

   Depositor

   Anna Huttenlocher

   Article

   Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

   Insert

   protein tyrosine phosphatase 1B (Ptpn1 Rat)
   

   Use

   Tags

   His and Xpress Epitope Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta1

   Plasmid

   #106114

   Purpose

   Lentiviral expression of PRKRA delta1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   PRKRA (PRKRA Human)
   

   Use

   Lentiviral

   Tags

   DDK (FLAG) and myc

   Expression

   Mutation

   AA 100-313

   Promoter

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-shTRAF2#2

   Plasmid

   #44128

   Depositor

   William Hahn

   Article

   Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

   Insert

   TRAF2 (TRAF2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

6. mOPN-PB0

   Plasmid

   #106449

   Purpose

   Bacterial expression of murine osteopontin

   Depositor

   Georg Weber

   Article

   Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

   Insert

   osteopontin (Spp1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. P-miR-145 (pTransluc-miR-145-1)

   Plasmid

   #21496

   Depositor

   Kenneth Kosik

   Article

   Xu et al Cell. 2009 May 15. 137(4):647-58.

   Insert

   1.5kb 5' of miR-145 (MIR145 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-IRES-hygro-EGFP-Stx17

   Plasmid

   #174652

   Purpose

   Vector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.

   Depositor

   Lukas Kapitein

   Article

   Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

   Insert

   EGFP-Stx17 (STX17 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. plentiCRISPR-sgGOT1

   Plasmid

   #72874

   Purpose

   The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgGOT1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Chx10 3kb AP (CC#318)

    Plasmid

    #15205

    Depositor

    Connie Cepko

    Article

    Rowan et al Dev Biol. 2005 May 15. 281(2):240-55.

    Insert

    Chx10 3kb AP reporter (Vsx2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pMKO.1 GFP B56 gamma3 shRNA 14-1

    Plasmid

    #10681

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    PP2A B56 gamma3 shRNA (PPP2R5C Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta23

    Plasmid

    #106117

    Purpose

    Lentiviral expression of PRKRA delta23

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and myc

    Expression

    Mutation

    AA 1-127

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIS-krPAM2

    Plasmid

    #25457

    Depositor

    Richard Mains

    Article

    Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

    Insert

    PAM2 (Pam Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Exon A deleted (exon A extends from nt 1475 to n…

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pET16b-Antp

    Plasmid

    #86866

    Purpose

    Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

    Depositor

    Michael Chin

    Article

    Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

    Insert

    Linker-Antp
    

    Use

    Tags

    Antp

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV syn CCR6-eLOV-TEVcs-tTA

    Plasmid

    #173116

    Purpose

    Expresses CCR6 receiver construct in neurons

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    CCR6-eLOV-TEVcs-tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a_TIAL1

    Plasmid

    #106092

    Purpose

    bacterial protein expression of TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIAL1 (TIAL1 Human)
    

    Use

    Tags

    6xHis-thrombin and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p416GPD-PYK1

    Plasmid

    #34604

    Depositor

    Markus Ralser

    Article

    Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

    Insert

    PYK1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. Venus rGBD

    Plasmid

    #99015

    Purpose

    Used as a RhoA-GTP sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    rGBD (Rtkn Mouse)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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