We narrowed to 152,851 results for: DES

1. pTU1-A-SP44-mVenus-I

   Plasmid

   #163763

   Purpose

   Expresses Streptomyces codon-optimised mVenus-I

   Depositor

   Simon Moore, Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Insert

   mVenus-I
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   SP44

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSP1D1G1

   Plasmid

   #97325

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1G1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of G residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pALPS

   Plasmid

   #90996

   Purpose

   Lentiviral vector for expression of heterologous genes

   Depositor

   Jeremy Luban

   Article

   Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET12a-AL-09 FL

   Plasmid

   #47081

   Depositor

   Marina Ramirez-Alvarado

   Article

   McLaughlin et al Protein Sci. 2006 Jul;15(7):1710-22. Epu

   Insert

   AL-09 (IGKV3-20 Human)
   

   Use

   Tags

   ompT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSunS

   Plasmid

   #171672

   Purpose

   SunS expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDB364

   Plasmid

   #110275

   Purpose

   N-terminal 10-His DsbC TEV-peptide fusion

   Depositor

   Christopher Bahl

   Article

   Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   10-His, TEV cleavage peptide, and mature DsbC (i.…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV sgRNA-Notch1 #1 GFP

   Plasmid

   #106950

   Purpose

   Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   gRNA targeting Notch1 (Notch1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMDC32B-AtMIR390a-NbSu-2

   Plasmid

   #213400

   Purpose

   Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

   Insert

   Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-mGrn-myc

   Plasmid

   #178944

   Purpose

   Construct for expressing murine progranulin (Grn) in AAV

   Depositor

   Erik Roberson

   Article

   Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

   Insert

   Grn (Grn Mouse)
   

   Use

   AAV

   Tags

   Myc

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFvL118

    Plasmid

    #64752

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGDP4 tet(A)

    Plasmid

    #112887

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    tet(A)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Sequence was codon optimized for E. coli K12 usin…

    Promoter

    Plac

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEasyG1_nat

    Plasmid

    #184911

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. TNF-SBP-mCherry_puromycin

    Plasmid

    #65285

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTW081

    Plasmid

    #64755

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYZ_3WJrep_N19_switch

    Plasmid

    #132723

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N19_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV-PIG-miR-22

    Plasmid

    #64229

    Purpose

    Expresses miR-22 in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

    Insert

    miR22 (MIR22, MIR22HG Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLXEBR-pTF-hM3Dq

    Plasmid

    #114283

    Purpose

    TET-inducible expression of the hM3Dq protein

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    hM3Dq
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a Gx-NL

    Plasmid

    #169105

    Purpose

    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-MBD1

    Plasmid

    #47057

    Purpose

    Expresses methyl-CpG binding domain protein 1

    Depositor

    Huda Zoghbi

    Article

    Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

    Insert

    Methyl-CpG Binding Domain Protein 1 (MBD1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA-h-mmPtch1-FL-TagBFP

    Plasmid

    #120921

    Purpose

    Expresses BFP tagged Ptch1

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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