We narrowed to 11,295 results for: cat.1
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Plasmid#173193PurposeExpresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.DepositorInsertT7-TetO
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
TagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialPromoterT5Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC016-gPsp-AUG16_start_codon
Plasmid#233612PurposeExpression of guide RNA targeting the start codon of the Renilla luciferase gene for PspCas13bDepositorInsertgRNA targeting Renilla luciferase
UseCRISPRAvailable SinceJuly 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_R261A
Plasmid#202586PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Arginine 261 to AlanineAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-Cer-NCreIntN
Plasmid#160505PurposeExpresses NCreIntN, tagged with HA, in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/LoxTagsHAExpressionMammalianPromoterCMVAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP L138 gRNA
Plasmid#119133PurposegRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.DepositorInserteGFP L138 gRNA
UseCRISPRMutationNonePromoterU6Available SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPLP4HS-mCf
Plasmid#229988PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.DepositorTypeEmpty backboneTagsHis6 and Twin-Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2S-mCf
Plasmid#229986PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.DepositorTypeEmpty backboneTagsStrep-tag IIExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pELuc
Plasmid#118056PurposeGBPart - ELuc Luciferase CDSDepositorInsertELuc
UseSynthetic Biology; Domestication of dna parts for…Available SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0620
Plasmid#133506PurposeExpression of eGFP tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-IntCCCre
Plasmid#160506PurposeExpresses IntCCCre, tagged with FLAG, in mammalian cells in split intern-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/LoxTagsFLAGExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCM167.2-Venus-VF-hADAM10(aa1-213)
Plasmid#189554PurposeProtein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
TagsVFExpressionMammalianMutationcontains aa 1-213Available SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-SBP-DICER1
Plasmid#50558Purposepurification of Dicer1 from mammalian cellsDepositorAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHPK1212
Plasmid#8834DepositorInserttobacco vein mottling virus protease, catalytic domain
TagsMBPExpressionBacterialAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCL0978
Plasmid#252424PurposeMitochondria-tethered construct for the RAPID-Release approachDepositorInsertHistone H3.1 (H3C1 Human)
TagsEGFP-TVMVx2-FRB-OMP25ExpressionMammalianMutationTruncation: aa 1-56 onlyPromoterCMVAvailable SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCL1009
Plasmid#252430PurposeMitochondria-tethered construct for the RAPID-Release approachDepositorInsertHistone H3.1 (H3C1 Human)
TagsEGFP-TVMVx2-FRB-OMP25ExpressionMammalianMutationTruncation: aa 1-30 onlyPromoterCMVAvailable SinceMarch 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
ACE2-Mutant-C1
Plasmid#240604PurposeTo test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 1 located within the collectrin-like site on ACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFLAG tagExpressionMammalianMutationArginine (R) and Lysine (K) residues on ACE2-clus…PromoterCMVAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-SpyTag-(AffiHER2)x3
Plasmid#216281PurposeThree affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate CatcherDepositorInsertSnoopTag-SpyTag-(AffiHER2)x3
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, and SpyTagExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLT237
Plasmid#174300PurposeReplicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.DepositorInsertPolC homology arm
ExpressionBacterialMutationchanged Cysteine 669 to TyrosineAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH858
Plasmid#172176PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
ExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only