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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 4801 - 4820 of 6562 results

  3. PX406 Pasteurella multocida Cas9

    Plasmid
    #68703
    Purpose
    Pasteurella multocida Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pUDP293

    Plasmid
    #204228
    Purpose
    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA
    Depositor
    Jean-Marc Daran
    Publication
    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d
    Inserts
    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    Use
    Synthetic Biology
    Tags
    NLS
    Mutation
    codon optimized for S. cerevisiae
    Promoter
    Saccharomyces cerevisiae PGK1 and Saccharomyces c…
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    204228-plasmid-map-sequence-id-408527

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  7. pEMS1602

    Plasmid
    #29305
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple131 (MKI67 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Nov. 4, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29305-plasmid-map-sequence-id-13720

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  8. 3xFLAG-dCas9/pMSCVhyg

    Plasmid
    #134323
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134323-plasmid-map-sequence-id-430622

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  11. pX330-Flag-dSpCas9

    Plasmid
    #92113
    Purpose
    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92113-plasmid-map-sequence-id-197502

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  12. pHS1993

    Plasmid
    #239836
    Purpose
    OMEGAOff-KRK mammalian expression
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A-DNMT3L-OrufIscB-KRK-KRAB
    Tags
    HA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    CMV
    Available since
    July 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239836-plasmid-map-sequence-id-479108

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  13. XLone-BSD Cas13d P2A mCherry

    Plasmid
    #155183
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry
    Depositor
    Xiaoping Bao
    Publication
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasRx
    Use
    Unspecified; Piggybac transposon
    Tags
    NLS and P2A mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155183-plasmid-map-sequence-id-307948

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  14. pTE4999

    Plasmid
    #107555
    Purpose
    Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hFnCpf1(dead1)
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    D917A
    Promoter
    CMV
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    107555-plasmid-map-sequence-id-207200

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  15. pCMV NucleoBAS N354K-I372V

    Plasmid
    #63574
    Purpose
    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET
    Depositor
    Stan van de Graaf
    Publication
    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NucleoBAS N354K-I372V
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    N345K and I372V
    Promoter
    CMV
    Available since
    May 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid
    #197503
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197503-plasmid-map-sequence-id-386604

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Showing: 4801 - 4820 of 6562 results