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pZ8-Prp

Plasmid

#74065

Purpose

pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR

Depositor

Timothy Lu

Publication

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

Propionate inducible promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

prp

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRB1083

Plasmid

#71480

Purpose

Expresses VRER Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Publication

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VRER Cas9 variant

Use

CRISPR

Expression

Worm

Promoter

eft-3 (eef-1A.1)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Tol2_ISCro4_Insertion_Reporter

Plasmid

#251500

Purpose

Reporter plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and pDonor_ISCro4_(WT)_Donor_ECFP plasmid

Depositor

Martin Jinek

Publication

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 insertion reporter

Expression

Mammalian

Mutation

NA

Available since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-EGFP

Plasmid

#233208

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

Depositor

Alejo Rodriguez-Fraticelli

Publication

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-StayGold

Plasmid

#211360

Purpose

Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Publication

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Tags

10xHis-tag

Expression

Bacterial

Promoter

T7

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAK_EF1a_CasRx_Hygro

Plasmid

#134840

Purpose

Lentiviral expression of CasRx T2A Hygromycin

Depositor

Michael Yaffe

Insert

RfxCas13d

Use

CRISPR and Lentiviral

Tags

T2A Hygromycin

Expression

Mammalian

Promoter

EF1a

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra2

Plasmid

#184933

Purpose

Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2

Expression

Worm

Promoter

tag-168

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGT-Ah7

Plasmid

#122574

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah10

Plasmid

#122577

Purpose

MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

tetQ/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Publication

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Expression

Bacterial

Promoter

PL(HrtO)

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.716

Plasmid

#108518

Purpose

PBAD-YFP Reporter. Ara sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBAD-YFP

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK570

Plasmid

#110615

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pAJM.712

Plasmid

#108513

Purpose

PCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCymRC-YFP

Expression

Bacterial

Available since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDD379

Plasmid

#91834

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Type

Empty backbone

Use

CRISPR

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ZIKV_Trigger_32B

Plasmid

#75009

Purpose

Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

Depositor

James Collins, Alexander Green

Publication

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_32B

Expression

Bacterial

Promoter

T7

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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ZIKV_Trigger_27B

Plasmid

#75008

Purpose

Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

Depositor

James Collins, Alexander Green

Publication

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_27B

Expression

Bacterial

Promoter

T7

Available since

Feb. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pG1AK-mCherry

Plasmid

#71737

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Publication

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

mCherry

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Promoter

pRplsWT

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Publication

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-CD11bDARPin

Plasmid

#198295

Purpose

Produces PVCs retargeted with anti-CD11b DARPin F7

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-CD11bDARPin

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-truncated

Plasmid

#198286

Purpose

Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-truncated

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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