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Showing: 7261 - 7290 of 23361 results

  5. pLJM1-Flag-Rheb-4Rs

    Plasmid
    #165022
    Purpose
    expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)
    Depositor
    Ken Inoki
    Article
    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d
    Insert
    Rheb-4Rs (RHEB Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    change lysine residues on position 109, 135, 151,…
    Promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165022-plasmid-map-sequence-id-326717

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  12. pET-EIN-AlkBH5(66-292)

    Plasmid
    #125732
    Purpose
    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    AlkBH5(66-292) (ALKBH5 Human)
    Use
    Tags
    6xHis and EIN
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125732-plasmid-map-sequence-id-244619

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  15. LLP773_pLenti-6xHRBKET-sgRNA

    Plasmid
    #211766
    Purpose
    Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    pCMV and U6
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211766-plasmid-map-sequence-id-417396

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  18. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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  21. pDest-Sumo

    Plasmid
    #106980
    Purpose
    LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag
    Depositor
    Han Choe
    Article
    Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10
    Type
    Empty backbone
    Use
    Tags
    His8-Sumo
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    106980-plasmid-map-sequence-id-204719

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Showing: 7261 - 7290 of 23361 results