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pStrep-9xEGFP-N1

Plasmid

#122496

Purpose

Expression of Strep-tagged 9xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 9xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pStrep-10xEGFP-N1

Plasmid

#122497

Purpose

Expression of Strep-tagged 10xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 10xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pStrep-11xEGFP-N1

Plasmid

#122498

Purpose

Expression of Strep-tagged 11xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 11xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pStrep-12xEGFP-N1

Plasmid

#122499

Purpose

Expression of Strep-tagged 12xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 12xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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SK-YFP-NM-A

Plasmid

#48664

Purpose

Bacterial NM repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

NM prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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SK-YFP-TD-A

Plasmid

#48666

Purpose

Bacterial TD repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V134L Cas2

Plasmid

#80092

Purpose

Encodes Cas1 V134L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V134L

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V33A Cas2

Plasmid

#80093

Purpose

Encodes Cas1 V33A and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V33A

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V190I Cas2

Plasmid

#80094

Purpose

Encodes Cas1 V190I and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V190I

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P202Q Cas2

Plasmid

#80096

Purpose

Encodes Cas1 P202Q and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P202Q

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P212L Cas2

Plasmid

#80097

Purpose

Encodes Cas1 P212L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P212L

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P282L Cas2

Plasmid

#80098

Purpose

Encodes Cas1 P282L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P282L

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P294H Cas2

Plasmid

#80099

Purpose

Encodes Cas1 P294H and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P294H

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 Cas2 T72I

Plasmid

#80100

Purpose

Encodes Cas1 and Cas2 T72I

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

T72I

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 Cas2 G74A

Plasmid

#80101

Purpose

Encodes Cas1 and Cas2 G74A

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

G74A

Availability

Academic Institutions and Nonprofits only

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Human Lipid Droplet and Metabolism Library

Pooled Library

#191535

Purpose

Targets 1,196 genes related to lipid droplet biology and cell metabolism.

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only
pCambia1300-PacI

Plasmid

#44183

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

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p426-SNR52p-gRNA.CAN1.Y-SUP4t

Plasmid

#43803

Purpose

Encodes a gRNA that targets Can1.Y in yeast.

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

CAN1.y gRNA (CAN1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 21, 2013

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-L-tetR

Plasmid

#62562

Purpose

Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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