We narrowed to 7,903 results for: nls

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pcDNA3.1-GoldenGate-VP64

Plasmid

#47389

Purpose

Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

Type

Empty backbone

Use

TALEN

Tags

FLAG, HA, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1209

Plasmid

#44491

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]

Use

Gateway expression clone

Available Since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1208

Plasmid

#44490

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]

Use

Gateway expression clone

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCT

Plasmid

#60620

Purpose

Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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3xFN-Tel-TAL/pCMV-7.1

Plasmid

#63589

Purpose

Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

Insert

3xFVN-Tel-TAL

Use

TALEN

Tags

3xFLAG-tag, V5-tag, and nuclear localization sign…

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DKAR-Nuc

Plasmid

#45499

Depositor

Alexandra Newton

Article

Bossuyt et al J Biol Chem. 2011 Sep 23;286(38):33390-4

Insert

DKAR-Nuc

Tags

CFP, NLS, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
JW616

Plasmid

#64805

Purpose

CUC2 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

GAL4 Activation Domain, HA, and SV40 NLS

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCGS652

Plasmid

#62220

Purpose

Mammalian expression TALEN vector with N152, C+18 truncations

Depositor

Daniel Voytas

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Type

Empty backbone

Use

TALEN

Tags

AcV5 and SV40 NLS

Expression

Mammalian

Promoter

miniCaggs

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98-ires-GFP

Plasmid

#237481

Purpose

Express NUP98 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98 (NUP98 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CCLMPC_HA-NUP98

Plasmid

#237482

Purpose

Express HA-tagged NUP98 in dual promoter lentiviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

HA-NUP98 (NUP98 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

MND/PGK

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-SaCas9-U6-sgDaglb

Plasmid

#240023

Purpose

Knockdown expression of Daglb

Depositor

Huaibin Cai

Article

Liu et al Nat Commun. 2022 Jun 17;13(1):3490. doi:

Insert

diacylglycerol lipase, beta (Daglb Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-SaCas9-U6-sgDagla

Plasmid

#239421

Purpose

Knockdown expression of Dagla

Depositor

Huaibin Cai

Article

Liu et al Nat Commun. 2022 Jun 17;13(1):3490. doi:

Insert

diacylglycerol lipase, alpha (Dagla Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKAREN5-gl

Plasmid

#225958

Purpose

The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-gl

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ167

Plasmid

#69309

Purpose

Gateway entry vector with Cas9p

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

Cas9p (Plant codon-optimized; high GC content at 5 prime region)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pPP537

Plasmid

#158803

Purpose

pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Hygro-PIP-FUCCI

Plasmid

#138714

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Nuclear PancACe

Plasmid

#215706

Purpose

Expresses acetyl-CoA biosensor in the nucleus

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-L

Plasmid

#59025

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-L

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-R

Plasmid

#59026

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-R

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-PIP-FUCCI

Plasmid

#138715

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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