We narrowed to 15,989 results for: LEA

1. pcKv10.1

   Plasmid

   #85703

   Purpose

   mammalian expression Kv10.1

   Depositor

   Luis Pardo

   Article

   Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

   Insert

   KCNH1 (KCNH1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP1673

   Plasmid

   #65769

   Purpose

   Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ220-Ultra

   Plasmid

   #176889

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   AsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 puro_shTBK1_3185

   Plasmid

   #167294

   Purpose

   Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   shRNA TBK1 (TBK1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRevTRE MKL1

   Plasmid

   #19846

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pFloat.T7_v1

   Plasmid

   #133070

   Purpose

   Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

   Depositor

   Kacper Rogala, David Sabatini

   Article

   Rogala et al Science. 2019 Oct 25;366(6464):468-475.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   3C-His6 and His6-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19/NLS-mChe

   Plasmid

   #183162

   Purpose

   Construct for transient expression of cell nucleus-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   NLS-mCherry
   

   Tags

   16 aa nuclear localization signal and 16 aa nucl…

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACS131

   Plasmid

   #204596

   Purpose

   Histone H3.1K27M-V5

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   histone H3.1 K27M (H3C1 Human)
   

   Use

   Lentiviral

   Tags

   human histone H3.1-V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

   Plasmid

   #87694

   Purpose

   Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   ERT2-PhoCl-Cre-PhoCl-ERT2
   

   Expression

   Mammalian

   Promoter

   Chicken beta actin

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA-trp-HYB

    Plasmid

    #64331

    Purpose

    trp1 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of trp1 deletion gRNA cassette
    

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pH-mono-MAD7

    Plasmid

    #170134

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    maize Ubiquitin-1, switchgrass Ubiquitin-1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV4-NIK-HA

    Plasmid

    #27554

    Depositor

    Shao-Cong Sun

    Article

    Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

    Insert

    NIK (MAP3K14 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    NIK starts at amino acid 3; C52R and R139H mutati…

    Promoter

    CMV

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pME-nls-sfGFP

    Plasmid

    #132973

    Purpose

    full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    nuclear localized super folder GFP
    

    Use

    Gateway vector

    Tags

    6x myc and SV40 NLS

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2T-CMV-eA3A-BE5-NG-BlastR

    Plasmid

    #152998

    Purpose

    High purity C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

    Plasmid

    #182390

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    OmpA-mCherry
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS613_pTetR-P2A-BFPnls/sgTelo

    Plasmid

    #108649

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Telomere-targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET41a iGluu

    Plasmid

    #119834

    Purpose

    Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluu
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S72T

    Promoter

    T7

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-CD9-(TEV)-tTA

    Plasmid

    #162596

    Purpose

    human CD9-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD9 (CD9 Human)
    

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and hU6

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. dTomato_LD 0_CS M_tTA-BFP

    Plasmid

    #58875

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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