We narrowed to 33,695 results for: Sis

1. FUW-MDmut-mTurquoise

   Plasmid

   #193095

   Purpose

   constitutive expression of a mutated (mut, R42A), non-functional version of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   MDmut-mTurquoise
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLVX-CALPAIN-7-mCherry, F98D

   Plasmid

   #180653

   Purpose

   Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMA5460

   Plasmid

   #184855

   Purpose

   Encodes G418 resistance and PhiC31-excisable hTFAM gene

   Depositor

   Mikhail Alexeyev

   Article

   Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

   Insert

   TFAM (TFAM Human)
   

   Use

   Retroviral; Phic31 attp-attb recombination/excisi…

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   LTR

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMA4000

   Plasmid

   #184857

   Purpose

   Cre-excisable TFAM in Blasticidin-resistannce retroviral vector

   Depositor

   Mikhail Alexeyev

   Article

   Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

   Insert

   TFAM (TFAM Human)
   

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CDS12_Cas9-NLS

   Plasmid

   #136122

   Purpose

   Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_AtcoCas9-NLS(noStop)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBtPIPLC_N168C His

   Plasmid

   #173809

   Purpose

   Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Mary F Roberts, Anne Gershenson

   Article

   He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

   Insert

   Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Asn168Cys for labeling

   Promoter

   T7

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HBT-CPK32ac-Flag

   Plasmid

   #107431

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK32 (CPK32 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Antares-SEPLuc C1A4E

   Plasmid

   #141088

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEP-C1A4E
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Please see depositor comments

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p5E-161K enhancer-NR2F2-MUT

   Plasmid

   #138371

   Purpose

   Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   161k human enhancer with conserved NR2F2 binding sites mutated
   

   Use

   Gateway compatible

   Tags

   Expression

   Mutation

   NR2F2 sites mutated

   Promoter

   none

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p5E-161K enhancer-ETS-CORE-MUT

    Plasmid

    #138372

    Purpose

    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    human 161K human enhancer with ETS bind sites mutated
    

    Use

    Gateway compatible

    Tags

    Expression

    Mutation

    ETS factor binding sites mutated

    Promoter

    NONE

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hHSF4b K294R

    Plasmid

    #118357

    Purpose

    This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K294R

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Gal4-HSF4b WT

    Plasmid

    #118358

    Purpose

    This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHyg-AID(31-114)

    Plasmid

    #99514

    Purpose

    Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(31-114) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    Expression

    Mutation

    internal inversion of nucleotides 265-312 of the …

    Promoter

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMCh-1474

    Plasmid

    #82420

    Purpose

    Plasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 NED
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 1159-1698 from nvGW182

    Promoter

    CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMCh-1475

    Plasmid

    #82421

    Purpose

    Plasmid for expression of HA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-1158 from nvGW182

    Promoter

    CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMCh-1476

    Plasmid

    #82422

    Purpose

    Plasmid for expression of NHA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 NED
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 1159-1698 from nvGW182

    Promoter

    CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMCh-1477

    Plasmid

    #82423

    Purpose

    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-1158 from nvGW182

    Promoter

    CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMCh-1479

    Plasmid

    #82425

    Purpose

    Plasmid for expression of GST-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 NED
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    deleted amino acids 1159-1698 from nvGW182

    Promoter

    EF1A

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMCh-1480

    Plasmid

    #82426

    Purpose

    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted

    Promoter

    EF1A

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR-IAA1_no stop

    Plasmid

    #83847

    Purpose

    Gateway donor vector for IAA1 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA1 (IAA1 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge