We narrowed to 8,227 results for: sars

1. pCag FlpO-2A-Cre EV

   Plasmid

   #129419

   Purpose

   episomal expression of FlpO and Cre recombinases

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-K-RAS V12

   Plasmid

   #31200

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   K-Ras (KRAS Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   G12V constitutively active

   Promoter

   Available since

   June 24, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

   Plasmid

   #125713

   Purpose

   Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Has Service

   AAV1 and AAV5

   Insert

   eOPN3
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 331-429

   Promoter

   hSyn1

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

   Plasmid

   #135814

   Purpose

   Fluorescent reporter for knock-in of TdTomato into the TH locus

   Depositor

   Tim Ahfeldt, Lee Rubin

   Article

   Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

   Insert

   TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
   

   Use

   Tags

   p2a-TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PGK-RAP1A-WT-puro

   Plasmid

   #156166

   Purpose

   Lentiviral vector for expression of RAP1A-WT, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-GATA4

   Plasmid

   #101417

   Purpose

   Donor Vector containing GATA4 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1 Sox2 3H b

   Plasmid

   #26352

   Depositor

   Matthew Meyerson

   Article

   Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

   Insert

   Sox2 shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

8. OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52379

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   OCT4

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-HNF1B-WT-Flag-UTR

   Plasmid

   #183239

   Purpose

   Expression of FLAG tagged HNF1B

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   HNF1 homeobox B (HNF1B Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-MPP8-10xHis

    Plasmid

    #194178

    Purpose

    Expresses MPP8-10xHis tagged

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

    Plasmid

    #160567

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINTO-N3::hTET3 HxD

    Plasmid

    #136365

    Purpose

    Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Use

    Tags

    2xStrepTagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    HxD (H1077Y, D1079A)

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-FOXN1

    Plasmid

    #101445

    Purpose

    Donor Vector containing FOXN1 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    FOXN1 (FOXN1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAD-CMV-Caveolin1-CMV-GFP

    Plasmid

    #83272

    Purpose

    Adenoviral expression of Caveolin1 with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Fernandez-Rojo et al Cell Rep. 2013 Jul 25;4(2):238-47. doi:

    Inserts

    CAV1 (CAV1 Human)
    eGFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastbac-Flag-BRCA1

    Plasmid

    #223228

    Purpose

    Insect expression construct for Flag-tagged BRCA1 protein production and purification

    Depositor

    Weixing Zhao

    Article

    Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

    Insert

    Breast Cancer 1 (BRCA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-ZNF804B

    Plasmid

    #88756

    Purpose

    Donor Vector containing ZNF804B transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF804B (ZNF804B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.23 C3_7

    Plasmid

    #60293

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    SLC30A8 enhancer (SLC30A8 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLenti-PGK-RAP1A-E63-puro

    Plasmid

    #156167

    Purpose

    Lentiviral vector for expression of RAP1A-E63, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Glutamine 63 to Glutamic acid

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-FOXQ1

    Plasmid

    #101628

    Purpose

    Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    FOXQ1 (FOXQ1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. shERK2b-mlpx-puro

    Plasmid

    #65230

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT2-shATRX

    Plasmid

    #124258

    Purpose

    Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shATRX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-PGK-flag-RAP1B-V12-puro

    Plasmid

    #156168

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-PGK-RAP1A-WT-neo

    Plasmid

    #156173

    Purpose

    Lentiviral vector for expression of RAP1A-WT, with neomycin section

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-CMV-PdCO-EGFP

    Plasmid

    #198512

    Purpose

    Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-PGK-RAP1A-E63-neo

    Plasmid

    #156174

    Purpose

    Lentiviral vector for expression of RAP1A-E63, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Glutamine 63 to Glutamic acid

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. shERK2a-mlpx-puro

    Plasmid

    #65229

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. shERK1a-mlpx-puro

    Plasmid

    #65228

    Purpose

    encodes a shRNA against ERK1

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENTR D-TOPO-C3_9

    Plasmid

    #60258

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    NKX6-1 enhancer (NKX6-1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL4.23 C3_25

    Plasmid

    #60305

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    ROCK1 enhancer (ROCK1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pLenti-PGK-RAP2A-V12-neo

    Plasmid

    #156178

    Purpose

    Lentiviral vector for expression of RAP2A-V12, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge