We narrowed to 15,822 results for: LEA

1. pCMV4-NIK-HA

   Plasmid

   #27554

   Depositor

   Shao-Cong Sun

   Article

   Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

   Insert

   NIK (MAP3K14 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   NIK starts at amino acid 3; C52R and R139H mutati…

   Promoter

   CMV

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

2. UBXD1-GFP

   Plasmid

   #86464

   Purpose

   Expression of human UBXD1 with C-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   UBXD1 (UBXN6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-AgdNK

   Plasmid

   #203226

   Purpose

   For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CMV-eA3A-BE5-NG-BlastR

   Plasmid

   #152998

   Purpose

   High purity C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_Pro-IL-1α

   Plasmid

   #214341

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL1A (IL1A Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS613_pTetR-P2A-BFPnls/sgTelo

   Plasmid

   #108649

   Purpose

   Telomere-targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Telomere-targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNOC-CRISPR-GFP

   Plasmid

   #100009

   Purpose

   Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-GFP

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

   Plasmid

   #132962

   Purpose

   Firefly-luciferase single-strand annealing assay cloning backbone

   Depositor

   Gang Bao

   Article

   Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

   Insert

   Firefly Luciferase
   

   Use

   CRISPR and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Stop sequence between luciferase gene

   Promoter

   CMV

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACEC3GH (Hyg)

   Plasmid

   #110084

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBAD-yhjH

    Plasmid

    #111613

    Purpose

    Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

    Depositor

    Tim Tolker-Nielsen

    Article

    Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

    Insert

    yhjH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A52V

    Promoter

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCer-TG2-YFP

    Plasmid

    #85471

    Purpose

    TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

    Depositor

    Ray Truant, Jeffrey Keillor

    Article

    Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

    Insert

    hTG2 (TGM2 Human)
    

    Use

    Tags

    EYFP and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. hJAM-A pcDNA3.1

    Plasmid

    #70073

    Purpose

    Expresses human junctional adhesion molecule-A in mammalian cells

    Depositor

    Terence Dermody

    Article

    Danthi et al J Virol. 2006 Feb;80(3):1261-70.

    Insert

    junctional adhesion molecule-A (F11R Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFYF1548 EMX1

    Plasmid

    #47508

    Purpose

    human gRNA expression vector targeting EMX1

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EMX1 (EMX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFloat.cspA_v1

    Plasmid

    #133071

    Purpose

    Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-CD63-(TEV)-tTA

    Plasmid

    #162597

    Purpose

    human CD63-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD63 (CD63 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. RPL13-ECFP-SNAP

    Plasmid

    #187967

    Purpose

    expresses RPL13 with C-terminal tags ECFP and SNAP

    Depositor

    Markus Wahl

    Article

    Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

    Insert

    RPL13 (RPL13 Human)
    

    Use

    Tags

    ECFP-SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hy_pMT Flag-URH49 WT

    Plasmid

    #110127

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    April 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNAintron-CHIKV-Envelope protein

    Plasmid

    #215700

    Purpose

    Produces the Chikungunya virus envelope protein E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV E3-E2-6K-E1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pEGFP-AID

    Plasmid

    #61926

    Purpose

    Expression of human AID tagged with EGFP

    Depositor

    Jon Houseley

    Article

    Lambert et al PLoS One. 2013 Dec 4;8(12):e82110. doi:

    Insert

    AICDA (AICDA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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