We narrowed to 83,971 results for: TRI

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UBQ10:sXVE:ER-S11-GFP1–9

Plasmid

#125666

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–9 fragment that can be used to detect a protein tagged with GFP10

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-S11-GFP1–9

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-AM

Plasmid

#153427

Purpose

Retroviral (MSCV) expression of ametrine fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

ametrine

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.34

Plasmid

#131370

Purpose

expression of GST:MEG-4 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-4 (C36C9.1 Nematode)

Expression

Bacterial

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-R251

Plasmid

#134307

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 R251A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-S245

Plasmid

#134302

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6p1 AO7R

Plasmid

#137842

Purpose

GST tagged AO7 encoding aa 126-216 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZBTB7B_2

Plasmid

#135747

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZBTB7B_1

Plasmid

#135746

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.33

Plasmid

#131369

Purpose

expression of GST:MEG-3 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-3 (meg-3 Nematode)

Expression

Bacterial

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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INPP5A / type I IP3 5-phosphatase from dog thyroid

Plasmid

#131767

Purpose

Expression in bacteria or COS-7 cells

Depositor

Christophe Erneux

Article

Verjans et al Biochem J. 1994 May 15;300 ( Pt 1):85-90

Insert

INPP5A

Expression

Bacterial

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pQlinkH-YbATP-grasp

Plasmid

#129639

Purpose

Expression plasmid of ATP-grasp from Y. bercovieri

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbATP-grasp

Tags

His6-tag

Expression

Bacterial

Promoter

pQE

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.31

Plasmid

#131371

Purpose

expression of GST:MEG-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-1 (meg-1 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG LacZ

Plasmid

#83093

Purpose

Gateway entry vector for inducible expression of LacZ with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Entry vector for gateway cloning

Tags

3xFLAG

Promoter

TRE tight promoter

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Promoter

tac promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_S1966C

Plasmid

#98681

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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p415TEF-NQYFP

Plasmid

#99554

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3_zfh1_CP-candidate_luc+

Plasmid

#86391

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only
pUAST attB BmOr2 (Y464A)

Plasmid

#73927

Purpose

UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to Alanine

Depositor

Leslie Vosshall

Article

Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

Insert

BmOr2 (Y464A)

Expression

Insect

Mutation

changed 464 Tyrosine to Alanine

Promoter

hsp70

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJK359

Plasmid

#71570

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctA)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX1ZFCNGA5

Plasmid

#69859

Purpose

GST tagged protein for antibody production screening

Depositor

Gary Matthews

Article

Khan et al Neuroscience. 2010 Jan 13;165(1):79-89.

Insert

ZFCNGA5, 3' end only with native stop (cnga5 Zebrafish)

Tags

GST

Expression

Bacterial

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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