We narrowed to 19,001 results for: Tec
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pUCBB-ctH6-eGFP
Plasmid#32562DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin sitePromoterConsitutive lacAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBT44c
Plasmid#138511PurposeP2DepositorInsertinteinC-dSpCas9-UGI
UseSynthetic BiologyAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 tdAPX W41F
Plasmid#40863PurposeTandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).DepositorInserttandem dimer pea APX W41F
TagsFlagExpressionMammalianMutationW41F mutationPromoterCMVAvailable SinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIS002
Plasmid#194013PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex3 XTR f1
ExpressionBacterialAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pT7-eGFP
Plasmid#32552DepositorAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVH607-Tier1-PCREm4-Nluc
Plasmid#169594PurposeTier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).DepositorInsertcAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4Available SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)
Plasmid#191139PurposeExpression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gatesDepositorInsertt17d9b
ExpressionMammalianMutationWTPromoterCMV-TOAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTK312
Plasmid#59569PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak EGFP ochre attB2 AscI
Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
GWF058
Plasmid#133609PurposeUAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPRDepositorInsertGal4DBD-NS3a, DNCR2-VPR
ExpressionMammalianAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58
Plasmid#171432PurposeExpression of hH3-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-hH3
ExpressionMammalianAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSPlit-OC-hDib1
Plasmid#58789PurposeExpress hDib1-LucC fusion protein in mammalian cellsDepositorAvailable SinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fluorescent reporter for MOBE1-2
Plasmid#219945PurposeMammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2DepositorInsertCMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
UseCRISPRExpressionMammalianMutationEGFP(A111V/L202S)PromoterCMV, U6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTNSQCas
Plasmid#219434PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInsertTnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
ExpressionBacterialAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-LoxP
Plasmid#219436PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInserttwo transposon ends, LoxP-terminator-LoxP gene cassette
ExpressionBacterialAvailable SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVH231-Tier1-PmPGK1-mTagBFP2
Plasmid#169520PurposeTier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).DepositorInsertPPGK-driven monomeric Tag blue fluorescent protein 2
ExpressionMammalianPromoterPPGKAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_SNAP
Plasmid#82716PurposeExpresses Myosin VI HMM with a C-terminal SNAP tag.DepositorInsertMyosin VI
Tags6xHis Tag, FLAG purification tag, and SNAP tagExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBJ5
Plasmid#167134PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterFrr promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
A3A-Gam
Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only
