We narrowed to 12,019 results for: pcdna

1. pcDNA GAL4 CREB 1-341

   Plasmid

   #22972

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

   Insert

   CREB
   

   Use

   Tags

   GAL4 DNA-binding domain

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5/FRT/TO HIS SSA1

   Plasmid

   #19543

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   SSA1 (SSA1 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-PKA-RIIβ-sREACh

   Plasmid

   #114113

   Purpose

   Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/Puro/FA/FFT-LL5BIP

   Plasmid

   #112830

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

   Insert

   LL5BIP (2410002F23Rik Mouse)
   

   Use

   Tags

   2xFlag-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H

   Plasmid

   #108376

   Purpose

   FRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 Flag Rab1a N124I

   Plasmid

   #46778

   Purpose

   expression of N124I Rab1a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab1a (Rab1a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N124I cannot stably bind guanine nucleotide

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-HA-RIPK3 2KR(55,363)

   Plasmid

   #78811

   Purpose

   To overexpress RIPK3 2KR(55,363) in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K55R, K363R

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/FRT-ChemoB-NAD

   Plasmid

   #193824

   Purpose

   CMV overexpression of ChemoB-NAD in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoB-NAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EBFP2:N39Y, A206K, T225R; HT7: L271E

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_AVPR2-NTEV-TCS-GV-2xHA

   Plasmid

   #194370

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   AVPR2-NTEV-TCS-GV-2xHA (AVPR2 Human, Synthetic)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-DDX11-Flag-KAE

    Plasmid

    #120728

    Purpose

    Expression of a Flag-tagged version of DDX11 KAE mutant in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 KAE mutant (DDX11 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Substitution of E201 and Y202 with K and A

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FRB-LplA(W37I)

    Plasmid

    #34840

    Depositor

    Alice Ting

    Article

    Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

    Insert

    FRB-LplA(W37I)
    

    Use

    Tags

    FLAG (internal fusion), HA, and His Tag

    Expression

    Mammalian

    Mutation

    LplA has Trp37 to Val mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-GFP-LplA(AAG)

    Plasmid

    #61820

    Purpose

    Contains GFP tag for direct live-cell fluorescence imaging of the resofurin ligase

    Depositor

    Alice Ting

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

    Insert

    E. coli lipoic acid ligase
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    E20A, F147A, H149G

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_Lyn11-STf

    Plasmid

    #182008

    Purpose

    CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    Lyn11-STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO/Flag-mKO2

    Plasmid

    #110371

    Purpose

    Mammalian expression of mKO2 with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    monomeric Kusabira Orange 2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid

    #157699

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pcDNA6 MCV LTco S147A

    Plasmid

    #112192

    Purpose

    Expression of MCPyV LT mutant (Ser 147 to Ala)

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCPyV LT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

    Plasmid

    #157692

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA3_HLA-cmyc-GRM3 C127A

    Plasmid

    #140451

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine

    Promoter

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-JNKsubDD-YPet-NES

    Plasmid

    #84633

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    JNKsubDD-YPet-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-hygro-eGFP-HEC1-9D

    Plasmid

    #114029

    Purpose

    Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9D (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 9D

    Promoter

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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