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pEMS2024

Plasmid

#49131

Purpose

AAV plasmid with hs671 promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-hs671-iCre

Use

AAV

Expression

Mammalian

Promoter

hs671

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2050

Plasmid

#49135

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pET22-HT/purC

Plasmid

#73623

Purpose

Produces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

phosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Mutation

Contains multiple silent mutations (E179, G185, G…

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase delta 55

Plasmid

#59824

Purpose

Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 55

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223 PAK5 P50L

Plasmid

#60533

Purpose

Donor vector for human Pak5 P50L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

P50L

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 P76T

Plasmid

#60534

Purpose

Donor vector for human Pak5 P76T mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

P76T

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S312C

Plasmid

#60535

Purpose

Donor vector for human Pak5 S312C mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

S312C

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 V463L

Plasmid

#60536

Purpose

Donor vector for human Pak5 V463L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

V463L

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 V593I

Plasmid

#60539

Purpose

Donor vector for human Pak5 V593I mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

V593I

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-hRIP1-FL I541P

Plasmid

#61381

Purpose

Expresses Human full length RIPK1 with I541P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP1with I541P mutation (RIPK1 Human)

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

changed I541 to P

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
tdEos-Actin-7

Plasmid

#57570

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Betzig et al Science. 2006 Sep 15;313(5793):1642-5. E

Insert

Actin (ACTB Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mKO-Vinculin-23

Plasmid

#57852

Purpose

Localization: Focal Adhesions, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Vinculin (VCL Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Frmd4b

Plasmid

#45867

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Frmd4b in situ probe (Frmd4b Mouse)

Use

In situ

Mutation

contains bp# 3933-4535 of NM_145148.2

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Lpl in situ probe

Plasmid

#45630

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Lpl in situ probe (Lpl Mouse)

Use

In situ

Mutation

fragment contains bp# 3249-3900 of NM_008509.2

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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Cterminal HA WDR59 pRK5

Plasmid

#46328

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR59 (WDR59 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-hCas3

Plasmid

#134920

Purpose

Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas3 with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA_MET_D14_3Flag

Plasmid

#182495

Purpose

Contains cDNA of the MET gene without the exon 14

Depositor

Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

MET (MET Human)

Tags

C-terminal 3xFlag tag

Expression

Mammalian

Mutation

Exon 14 deleted

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX_305-N-dTAG

Plasmid

#91797

Purpose

Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

hPGK

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pWF073_LVt-EF1a-eGFP-ffLuc-PGK-BlastR

Plasmid

#233447

Purpose

Stable expression of eGFP-firefly luciferase (ffLuc)

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Insert

eGFP-firefly luciferase (ffLuc)

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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