We narrowed to 14,090 results for: cas9

1. pKER-mCardinal

   Plasmid

   #85976

   Purpose

   Expresses mCardinal from the EF1a promoter in mammalian cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   mCardinal
   

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET42b-AncBE4max

   Plasmid

   #165157

   Purpose

   Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression

   Depositor

   Taekjip Ha

   Article

   Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

   Insert

   AncBE4max
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA6213S

   Plasmid

   #122094

   Purpose

   constitutive expression of I-SceI for genome engineering in Pseudomonas

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Insert

   I-SceI restriction endonuclease
   

   Promoter

   Pem7

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF525-AcrIIA4_gb39_lenti

   Plasmid

   #138305

   Purpose

   Lenti expression of AcrIIA4

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGNW2

   Plasmid

   #122086

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJAT32

   Plasmid

   #204297

   Purpose

   pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LBJJ383

   Plasmid

   #117517

   Purpose

   PCR template for sgRNA classic with polyT as terminator

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6-26

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUGW NLS-FlpO

   Plasmid

   #183448

   Purpose

   Lentiviral FlpO vector

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   NLS-FlpO
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. BFP dest clone

   Plasmid

   #71825

   Purpose

   Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Cry2FL-VP64

    Plasmid

    #60554

    Purpose

    Expresses Cry2FL-VP64 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    Cry2FL-VP64
    

    Tags

    FLAG, HA tag, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAT39

    Plasmid

    #204289

    Purpose

    For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-EGFP+H3:H26
    

    Use

    CRISPR

    Promoter

    MHC

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-Basic-8x-gRNA-eGFP

    Plasmid

    #60718

    Purpose

    eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Promoter

    TgTUB1 and Toxoplasma U6

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330_SYCP3_nterm

    Plasmid

    #222524

    Purpose

    Cas9/sgRNA plasmid for targeting SYCP3

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    Cas9, SYCP3 sgRNA (SYCP3 Synthetic, Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. YN2_1_LT58_X2site

    Plasmid

    #177705

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBGK

    Plasmid

    #65796

    Purpose

    Vector for Cas9 mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Harms et al Curr Protoc Hum Genet. 2014 Oct 1;83:15.

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH375

    Plasmid

    #86841

    Purpose

    mamalian expression of anti-CRISPR protein AcrIIA3

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGNW4

    Plasmid

    #122088

    Purpose

    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBFv-U6.2B

    Plasmid

    #138401

    Purpose

    gRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Insert

    U6-2
    

    Expression

    Insect

    Promoter

    U6-2

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJAT77

    Plasmid

    #204295

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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