We narrowed to 12,275 results for: cel.2

1. pNKX2-5-GFP-T2A

   Plasmid

   #203608

   Purpose

   Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

   Depositor

   Matthew Birket

   Article

   Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

   Insert

   NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)
   

   Use

   Human targeting

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-sgRNA_Ago1_4

   Plasmid

   #73536

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pLOVE-Sox2

   Plasmid

   #15953

   Depositor

   Miguel Ramalho-Santos

   Article

   Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

   Insert

   SRY (sex determining region Y)-box 2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   The recombination reaction required to clone &quo…

   Available Since

   Nov. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.TRC1.shmNsd1.2, puro

   Plasmid

   #114447

   Purpose

   Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

   Depositor

   Adrian Bracken

   Article

   Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

   Insert

   shNsd1.2 (Nsd1 Mouse)
   

   Use

   Lentiviral and RNAi

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.TRC1.shmNsd1.1, puro

   Plasmid

   #114446

   Purpose

   Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

   Depositor

   Adrian Bracken

   Article

   Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

   Insert

   shNsd1.1 (Nsd1 Mouse)
   

   Use

   Lentiviral and RNAi

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. zebrafish similar to gpr151 (or gpcr-2037)_L (OZ535)

   Plasmid

   #27212

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   Feb. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

7. zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536)

   Plasmid

   #27213

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-Syn-ERT2-iCre-ERT2

   Plasmid

   #178059

   Purpose

   Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ERT2-iCre-ERT2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human synapsin 1 (Syn) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1-IL6

   Plasmid

   #111933

   Purpose

   Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

   Insert

   Interleukin 6 (IL6 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   CMV

   Available Since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST40-2XFL-Src-KD

    Plasmid

    #140313

    Purpose

    Expresses Flag-tagged human Src-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Contains only the KD region

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FASTHDR-Cterm-mClover3-Puromycin

    Plasmid

    #167205

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mClover3

    Expression

    Mammalian

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FASTHDR-Cterm-HaloTag-Puromycin

    Plasmid

    #167208

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac Dual α1B/β2B Tubulin

    Plasmid

    #170553

    Purpose

    Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB2B (TUBB2B Human)
    

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

    Plasmid

    #158953

    Purpose

    Express human RAD51(T309A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed threonine 309 to alanine

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UBC-ERT2-iCre-ERT2

    Plasmid

    #178060

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. FASTHDR-Cterm-mRuby3-Zeo

    Plasmid

    #167206

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mRuby3

    Expression

    Mammalian

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. FASTHDR-Cterm-mTagBFP2-Blasticidin

    Plasmid

    #167207

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mTagBFP2

    Expression

    Mammalian

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

    Plasmid

    #158952

    Purpose

    Express human RAD51(F129A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed phenylalanine 129 to alanine

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST40-2XFL-Src-S4-KD

    Plasmid

    #140297

    Purpose

    Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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