We narrowed to 9,379 results for: pcDNA 3.1

1. pcDNA-Cx43-S279/282D

   Plasmid

   #60714

   Purpose

   mammalian expression of rat Cx43 S279/282D mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Expression

   Mammalian

   Mutation

   S279/282D

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.2-Cx43-M100L-siResist

   Plasmid

   #49854

   Purpose

   Encodes human connexin 43 with M100 mutated to L and wobble mutations conferring resistance against an siRNA

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Expression

   Mammalian

   Mutation

   Methionine100 mutated to Leucine. Contains severa…

   Promoter

   CMV

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA-Cas9-T2A-STOP-TdT

   Plasmid

   #126425

   Purpose

   Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Mutation

   alanine-6 of TdT has been changed to a STOP codon

   Promoter

   pCMV

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-R536C-WLS-HA-IRES-GFP

   Plasmid

   #178065

   Purpose

   Expression of WLS-HA protein (R536C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   R536C

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Y478C-WLS-HA-IRES-GFP

   Plasmid

   #178063

   Purpose

   Expression of WLS-HA protein (Y478C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Y478C

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Y392C-WLS-HA-IRES-GFP

   Plasmid

   #178062

   Purpose

   Expression of WLS-HA protein (Y392C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Y392C

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-I531T-WLS-HA-IRES-GFP

   Plasmid

   #178064

   Purpose

   Expression of WLS-HA protein (I531T mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   I531T

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Cas9-T2A-TdT

   Plasmid

   #126424

   Purpose

   Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-SARS-CoV-2-S-RBD-Fc

   Plasmid

   #141183

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
   

   Tags

   Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-mTET2 CD mutant

    Plasmid

    #89896

    Purpose

    Expresses mouse TET2 CD mutant in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    CD (cysteine-rich, dioxygenase domain) truncation…

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mPC1_4077-eGFP-3HA

    Plasmid

    #108390

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA TMEM 138-flag

    Plasmid

    #41628

    Depositor

    Joseph Gleeson

    Article

    Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

    Insert

    Transmembrane Protein 138 (TMEM138 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

    Plasmid

    #114144

    Purpose

    The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
    

    Expression

    Mammalian

    Mutation

    G2A mutation and silent mutation to resist shRNA …

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-mPC1_4119-eGFP-3HA

    Plasmid

    #108393

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)

    Plasmid

    #155010

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    E1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-hPC1_4106-eGFP-3HA

    Plasmid

    #108399

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4106 to 4302

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-sACE2-T92Q-Fc(IgG1)

    Plasmid

    #145170

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-Flag-SPRTN-Y117C

    Plasmid

    #110216

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y117C, P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-sACE2-T92Q-sfGFP

    Plasmid

    #145178

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP glycosylation mutant T92Q

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-sACE2-T92Q-Fc(IgA1)

    Plasmid

    #145162

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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