We narrowed to 7,347 results for: GFP expression plasmids

1. pAWK61

   Plasmid

   #163642

   Purpose

   rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

   Depositor

   David Matus

   Article

   Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

   Insert

   DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Nematode, Human)
   

   Use

   CRISPR

   Tags

   2xmKate2 and GFP

   Expression

   Worm

   Promoter

   rps-27

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1592

   Plasmid

   #121059

   Purpose

   GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   GFP^SEC^BioTag::AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

   Expression

   Worm

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. miniTurbo-dCasRx

   Plasmid

   #219821

   Purpose

   EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)

   Depositor

   Philipp Maass

   Insert

   dCasRx
   

   Use

   CRISPR

   Tags

   miniTurbo

   Expression

   Mammalian

   Promoter

   EF1a and hU6

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Cre

   Plasmid

   #24064

   Purpose

   Retroviral coexpression of Cre and EGFP

   Depositor

   Dan Littman

   Insert

   Cre
   

   Use

   Cre/Lox and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   MESV 5' LTR

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24a-VHH-APEX2

   Plasmid

   #109420

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-VHH-mCherry

   Plasmid

   #109421

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET24a-VHH-myc

   Plasmid

   #109422

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, T7, and myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP777_SnoopCatcher-p65HSF1-CO

   Plasmid

   #211770

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SnoopCatcher-p65HSF1
   

   Tags

   2xOLLAS

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP776_SpyCatcher-p65HSF1-CO

   Plasmid

   #211769

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-p65HSF1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP783_SpyCatcher-DNMT3A-CO

    Plasmid

    #211776

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavudin-DNMT3A
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP784_SnoopCatcher-DNMT3A-CO

    Plasmid

    #211777

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Spy-EZH2
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLP780_SpyCatcher-KRAB-CO

    Plasmid

    #211773

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-KRAB
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP789_αGCN4-DNMT3A-CO

    Plasmid

    #211783

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-DNMT3A
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET24a-VHH-tev-APEX2

    Plasmid

    #117751

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLP782_Traptavidin-KRAB-CO

    Plasmid

    #211775

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-DNMT3A
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLP785_Traptavidin-DNMT3A-CO

    Plasmid

    #211778

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-EZH2
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP781_SnoopCatcher-KRAB-CO

    Plasmid

    #211774

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-KRAB
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas9FnCpf1GG

    Plasmid

    #131013

    Purpose

    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCellFree_KA1_SARS-COV-2_NSP3

    Plasmid

    #169378

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP3
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCellFree_KA1_SARS-COV-2_NSP2

    Plasmid

    #169377

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP2 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP2
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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