We narrowed to 19,551 results for: iNOS

1. Pet15b-Internalization and Endosomal Escape

   Plasmid

   #186551

   Purpose

   Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   10R-8His-ETA-EGFP
   

   Use

   Tags

   8xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Flag Nprl2 pLJM1

   Plasmid

   #46338

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Nprl2 (NPRL2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pMD19T-psba1-TetR-PL22-dCas9-SpR

   Plasmid

   #73223

   Purpose

   Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   dCas9 from S. pyogenes
   

   Use

   Tags

   c-myc

   Expression

   Bacterial

   Mutation

   Silent mutation at bp 1341 A->C to remove an E…

   Promoter

   PL22

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GST-MAGE-2-429

   Plasmid

   #89129

   Purpose

   bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

   Depositor

   Elizabeth Wilson

   Article

   Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

   Insert

   MAGE-A11 (MAGEA11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pSUPER.retro-{beta}4 siRNA

   Plasmid

   #16266

   Depositor

   Arthur Mercurio

   Article

   Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

   Insert

   SIRNA Beta 4 Integrin (ITGB4 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pET28a-Beclin1 CC

   Plasmid

   #24403

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains the CC domain (aa 151-242)

   Promoter

   Available Since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pJMP1335

   Plasmid

   #119269

   Purpose

   rfp "test" strain

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET29b-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175783

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNXgate32-3HA

   Plasmid

   #105076

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   3xHA and NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCH6 - pBait-ChiX

    Plasmid

    #174662

    Purpose

    arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

    Depositor

    Katherine Berry

    Article

    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

    Insert

    ChiX
    

    Use

    Tags

    1xMS2 hairpin

    Expression

    Bacterial

    Mutation

    Promoter

    pBad

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPD_Psra-6::ChR2(H134R)::mCherry

    Plasmid

    #117419

    Purpose

    For selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoter

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    channel rhodopsin-2 with H134R mutation
    

    Use

    Tags

    Expression

    Worm

    Mutation

    H134R

    Promoter

    sra-6

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SNF-5 pXOOM

    Plasmid

    #48697

    Purpose

    Expressed SNF-5 in Xenopus oocytes or mammalian cells

    Depositor

    Dmitri Boudko

    Article

    Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

    Insert

    snf-5 (snf-5 Nematode)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mammalian

    Mutation

    K42E (when compared to NP_496735.1)

    Promoter

    T7

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiCRISPRv2-dADAM17 (#1)

    Plasmid

    #177259

    Purpose

    A knockout vector for the dog Adam17

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

    Plasmid

    #79671

    Purpose

    Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

    Depositor

    Scott Sternson

    Article

    Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAT024

    Plasmid

    #171636

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BFP
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJMP1363

    Plasmid

    #119279

    Purpose

    for stabilizing ICE in the presence of rapI expression

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKmyc-Borg3

    Plasmid

    #141074

    Purpose

    Expresses Borg3 (Cdc42ep5) in mammalian cells

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol. 2001 Oct;3(10):861-6. doi

    Insert

    Borg3 (Cdc42ep5 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223 GABARAP WT

    Plasmid

    #123080

    Purpose

    Gateway entry clone encoding human GABARAP wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSAG1::CAS9-U6::sg290860-6

    Plasmid

    #59855

    Purpose

    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

    Depositor

    David Sibley

    Article

    Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

    Insert

    CRISPR sg290860-6
    

    Use

    CRISPR; ; toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RCAS DNhRARalpha (CC#1865)

    Plasmid

    #15153

    Depositor

    Connie Cepko

    Article

    Sen et al Development. 2005 Dec . 132(23):5147-59.

    Insert

    RAR alpha (dom neg) (RARA Human)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Dominant negative. Truncated at aa403, so lacks t…

    Promoter

    Available Since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only