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pCMV-miR21

Plasmid

#20381

Depositor

Bryan Cullen

Article

Zeng et al RNA. 2003 Jan . 9(1):112-23.

Insert

miR21 (MIR21 Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-Tlr4

Plasmid

#27148

Depositor

Bruce Beutler

Article

Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

Insert

toll-like receptor 4 (Tlr4 Mouse)

Tags

3x FLAG

Expression

Mammalian

Mutation

Native signal peptide sequence (original amino ac…

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV14-mULK1

Plasmid

#24301

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Tags

3xFLAG

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
TRF2-IRESS-eGFP

Plasmid

#19798

Depositor

Mark Mattson

Article

van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

Insert

TRF2 (TERF2 Human)

Expression

Mammalian

Mutation

When compared to GenBank reference sequence NP_00…

Promoter

CMV-F

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
GFP-Bcl2-Maob

Plasmid

#18001

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Mitochondria-targeted Bcl-2 (BCL2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Bcl-2's C terminal replaced with mitochondri…

Promoter

CMV

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

Plasmid

#50464

Purpose

Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

Depositor

Bryan Roth

Insert

hM4D(Gi)-IRES-mCitrine (CHRM4 Human)

Use

AAV

Tags

HA

Promoter

human Synapsin 1

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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ROSAFARY

Plasmid

#13446

Depositor

Philippe Soriano

Article

Chen et al Nat Genet. 2004 Mar . 36(3):304-12.

Insert

ROSAFARY

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetON-Sox10

Plasmid

#115242

Purpose

Lentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

SOX10 (SOX10 Human)

Use

Lentiviral

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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HA-EglN2-pcDNA3

Plasmid

#18961

Depositor

William Kaelin

Article

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 2 (EGLN2 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His Snk/Plk2

Plasmid

#16015

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Tags

V5-His

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
1432 pcDNA3 flag MAGI1c

Plasmid

#10714

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

MAGI-1 (Magi1 Mouse)

Tags

flag

Expression

Mammalian

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Luciferase

Plasmid

#99283

Purpose

All-in-one lentivector with tet-inducible luciferase

Depositor

Stephen Tapscott

Article

Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

Insert

Firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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TIGRE docking site targeting vector

Plasmid

#61580

Purpose

Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Type

Empty backbone

Use

Mouse Targeting

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pBY2982

Plasmid

#23220

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

BirA-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 Wt (1-674aa)

Plasmid

#75478

Purpose

mammalian expression of GADD34

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pCBFRE-(mt)-luc

Plasmid

#26896

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated CBF1 sites (see note)

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACE2(WT)-8his

Plasmid

#149268

Purpose

Mammalian expression plasmid for his-tagged sACE2

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Short GSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBabepuro3-p16Flag

Plasmid

#24934

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p16 (CDKN2A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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