We narrowed to 16,817 results for: CAG

1. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216249

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgRANGAP.C1 (RANGAP1 Human)
   

   Use

   CRISPR; Endogenous tagging

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

   Plasmid

   #180153

   Purpose

   td-sfGFP dre reporter with Rosa26 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   td-sfGFP
   

   Use

   Mouse Targeting; Dre/rox

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

   Plasmid

   #104122

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   spine-jRGECO1a-P2A-QuasAr-dark Citrine
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

   Plasmid

   #196890

   Purpose

   Neuron-specific expression of human (h)Centrin fused to mNeonGreen

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mNeonGreen-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

   Plasmid

   #172479

   Purpose

   tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

   Depositor

   Mario Capecchi

   Insert

   tdTomato
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

   Plasmid

   #114094

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-RR (S542R/K607R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R and K607R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

   Plasmid

   #171965

   Purpose

   Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

   Insert

   Synthetic CLTX CD32a ITAM CD3z CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

   Plasmid

   #206115

   Purpose

   expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (Cacna1a Rat)
   

   Expression

   Mammalian

   Mutation

   E320A, E670A, E1411A, E1707A

   Promoter

   CMV/B-actin

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

   Plasmid

   #104566

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-p65
   

   Tags

   3x HA and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

    Plasmid

    #114726

    Purpose

    dCas9-VP160 expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    dCas9-VP160
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA

    Plasmid

    #196896

    Purpose

    Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    N-Cadherin-2xHA (Cdh1 Mouse)
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid

    #180151

    Purpose

    td-sfGFP cre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP

    Plasmid

    #196875

    Purpose

    Neuron-specific expression of LynGFP reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynEGFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid

    #114091

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R and S542R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Mutation

    D10A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. H11-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid

    #180157

    Purpose

    td-sfGFP dre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Mouse Targeting; Dre/rox

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid

    #180155

    Purpose

    td-sfGFP cre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

    Plasmid

    #233067

    Purpose

    Piggybac CRISPRi construct

    Depositor

    Markus Landthaler

    Article

    Vučićević et al bioRxiv 2024.09.12.612198

    Insert

    KRAB-dCas9
    

    Use

    CRISPR

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

    Plasmid

    #229827

    Purpose

    A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xnls-TagBFP2-MCS-FLAG
    

    Expression

    Mammalian

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-SNAP-p53DD-IRES-HRAS(G12V)-bGH

    Plasmid

    #233204

    Purpose

    AAV expression of SNAP-p53DD and HRAS(G12V)

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    SNAP-p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    G12V

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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