We narrowed to 7,945 results for: Lif;
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Plasmid#113088PurposeLuciferase reporter containing PAK4- FL-3'UTRDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseExpressionMammalianMutationnonePromoterPGKAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-PAX9
Plasmid#75079Purposeretroviral vector holding human PAX9 cDNADepositorInsertPAX9 (PAX9 Human)
UseRetroviralExpressionMammalianMutationwild-type, full-length, without native 3' UTRAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-hSt3Cas9 (pXK-1721)
Plasmid#208828PurposeCRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.DepositorTypeEmpty backboneUseCRISPR; Stcas9ExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSR (mCherry-surrogateTarget-bsr)
Plasmid#66950PurposeEncodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.DepositorInsertblasticidin
UseCRISPR, Mouse Targeting, and TALENExpressionMammalianPromoterbeta-actinAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW300ParsR-Amp32T
Plasmid#78652PurposepSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertaraC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
UseSynthetic BiologyPromotersee insertAvailable SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-CHAD
Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPO635
Plasmid#195297PurposepACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).DepositorInsertTnsABC operon of McCAST (Tn7575)
ExpressionBacterialPromoterIPTG inducible lac promoterAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH131
Plasmid#48260PurposeTemplate plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
GAL10/GAL1 bidirectional promoter
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterGAL10/GAL1 bidirectionalAvailable SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN-R2-mCsfGFP-L3
Plasmid#178602PurposeIt can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.DepositorInsertTandem fluorescent protein timers (tFTs)
UseEntry vector for gateway cloningTagsmCherry superfolder GFPAvailable SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3xflagGREB1b
Plasmid#112222Purposemammalian expression of flag tagged GREB1bDepositorAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
IpO
Plasmid#48233PurposeBase vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.DepositorInsertsURA3 3' fragment
URA3 5' fragment
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterURA3 promoter and noneAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
NET33 pmRFP-N2 (448)
Plasmid#61979Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPO636
Plasmid#195299PurposepACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).DepositorInsertTnsABCQ operon of McCAST (Tn7575)
ExpressionBacterialPromoterIPTG inducible lac promoterAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFG.CNa22_opt.IRES.eGFP
Plasmid#22491DepositorAvailable SinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTYF-1xGfaABC1D-tTA
Plasmid#19977DepositorInsertGfaABC1D-tTA (GFAP E.coli, Human)
ExpressionMammalianAvailable SinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
ERroGFP_C147S_pCDNA3
Plasmid#48630PurposeMammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)DepositorInsertERroGFP
TagsFLAGExpressionMammalianMutationC147SAvailable SinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIhh1.3K-Luc
Plasmid#53604PurposeReporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
C513-E07: attB5r-<p10p-polyhedrinp>-attB1r
Plasmid#162949PurposeGateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertdual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
ERrefGFP1_pCDNA3
Plasmid#48635PurposeMam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFPDepositorInsertERrefGFP1
TagsFLAGExpressionMammalianMutationC147S,C204QAvailable SinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only