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pHR-CMV-nlsCRE

Plasmid

#12265

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA+WT GADD45

Plasmid

#24929

Depositor

Xin Wang

Article

Yang et al J Biol Chem. 2000 Nov 24. 275(47):36892-

Insert

GADD45 (GADD45A Human)

Tags

HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pYPQ141D2.0

Plasmid

#99906

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-sgRNA-Sirius-8XMS2

Plasmid

#121942

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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2.2

Plasmid

#34885

Depositor

Irvin Chen

Article

Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

Insert

Sindbis virus envelope protein

Use

Lentiviral

Expression

Mammalian

Mutation

SGN UTR in E1 domain

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pYPQ141B2.0

Plasmid

#99897

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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mc2 mCherry

Plasmid

#206219

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAT9624-BEAR-cloning

Plasmid

#162986

Purpose

BEAR target cloning plasmid with split EGFP

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

exchangeable cassette

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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AMPK-γ1-HA

Plasmid

#40605

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-γ1 (Prkag1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pGEX6P1-hPREP

Plasmid

#179387

Purpose

Express human prolyl oligopeptidase (tagged) in bacterial cells

Depositor

Christian Gruber

Article

Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

Insert

human prolyl oligopeptidase (PREP Human)

Tags

GST

Expression

Bacterial

Promoter

TAC

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAC026

Plasmid

#227598

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

Depositor

Brittany Adamson

Article

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ER-mCherry-eMagA

Plasmid

#162248

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-mCherry-eMagA

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL90003

Plasmid

#68262

Purpose

Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6 (Triticum aestivum)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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CuGFP-ATG8(416)

Plasmid

#49423

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

Depositor

Daniel Klionsky

Article

Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

CUP1

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pQE-60 roGFP2-Orp1-His

Plasmid

#64976

Purpose

Bacterial expression of His-tagged roGFP2-Orp1

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Tags

His and roGFP2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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iRFP-PAmCherry1-PH_PLCd1

Plasmid

#92167

Purpose

PI(4,5)P2 single molecule fluorescent reporter

Depositor

Xuelin Lou

Article

Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

Insert

iRFP-PAmCherry1-PH_PLCd1

Tags

iRFP-PAmCherry1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-fNPY-GFP

Plasmid

#22912

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu neuropeptide Y promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pmScarlet_NES_C1

Plasmid

#85060

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKH142_EGFP-YIPF3 WT

Plasmid

#214974

Purpose

GFP tagged YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GFP-YIPF3 (YIPF3 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH116_keima-YIPF3

Plasmid

#214970

Purpose

Golgiphagy flux reporter keima-YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

keima-YIPF3 (YIPF3 Human)

Use

Lentiviral

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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