We narrowed to 13,665 results for: crispr cas9

1. pAGM65893

   Plasmid

   #153216

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. cr3Dd

   Plasmid

   #184862

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, bet, exo
   

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCold CL7-Cas12a

   Plasmid

   #124891

   Purpose

   obtaining full Cas12a RNP

   Depositor

   Yi Liu

   Article

   Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

   Insert

   Cas12a
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. cr3Vn

   Plasmid

   #184861

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, gam, bet, exo
   

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. cr3Ec

   Plasmid

   #184860

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, gam, bet, exo
   

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-Decoy

   Plasmid

   #80324

   Purpose

   Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Insert

   Decoy sgRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSECC

   Plasmid

   #60820

   Purpose

   3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

   Depositor

   Tyler Jacks

   Article

   Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

   Inserts

   Cas9
   Cre
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   BsmBI site eliminated by C->A; E308G

   Promoter

   EFS and EFS (after Cas9-2A)

   Available Since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

   Plasmid

   #196250

   Purpose

   Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL1-GlpT-scGFP-attL2
   

   Expression

   Bacterial

   Promoter

   J23119 promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. VVT1

    Plasmid

    #65779

    Purpose

    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SaCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. AP54-5

    Plasmid

    #65599

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APs12
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

12. AP56-6

    Plasmid

    #65593

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APa13
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

13. AP56-5

    Plasmid

    #65591

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APa13
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

14. AP56-8

    Plasmid

    #65596

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APa13
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

15. AP56-7

    Plasmid

    #65594

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APa13
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

16. AP54-3

    Plasmid

    #65597

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APs12
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pRDB_268

    Plasmid

    #231112

    Purpose

    SpG-Cas9

    Depositor

    John Doench, David Root

    Insert

    Cas9-SpG
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. P4 cosmid

    Plasmid

    #196335

    Purpose

    P4 cosmid with CRISPR-Cas9 system

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    genes from phage P4, CRISPR-Cas9 system
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS SK mCherryROSAegfp

    Plasmid

    #54322

    Purpose

    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and EGFP
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-sgRNA(MS2)

    Plasmid

    #102560

    Purpose

    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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