We narrowed to 535 results for: TRE3G

1. PB-tetO-NB

   Plasmid

   #197092

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL123

   Plasmid

   #187889

   Purpose

   AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9

   Depositor

   Martin Kampmann

   Article

   Leng et al Nat Neurosci. 2022 Nov;25(11):1528-1542.

   Insert

   NFIA-SOX9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Blasticidin XLone-eGFP

   Plasmid

   #159754

   Purpose

   AAVS1 donor plasmid for targeted inducible eGFP expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   all-in-one tet-on system
   

   Use

   CRISPR and TALEN; Donor plasmid for targeted knoc…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS inducible promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR

   Plasmid

   #204151

   Purpose

   Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   SARS-CoV-2 Delta variant spike
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   21 amino acid cytoplasmic tail deletion

   Promoter

   TRE3G

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTetON-DHFRdd-SpvB; CMV-mCherry

   Plasmid

   #89463

   Purpose

   Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Use

   Tags

   E. coli DHFRdd destabilization domain and mCherry…

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   TRE3G

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTetON-DeAct-GS1

   Plasmid

   #89454

   Purpose

   Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-GS1 (GSN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning cytoplasmic Gsn amino acids 5…

   Promoter

   TRE3G

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRE-BI-aaAFB2

   Plasmid

   #207839

   Purpose

   Inducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   atAFB2
   mCherry
   miniIAA7
   VHH-GFP4
   

   Use

   Tags

   3X FLAG-Tag and weak NLS

   Expression

   Mammalian

   Mutation

   All K mutated to R

   Promoter

   TRE3G BI promoter

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

   Plasmid

   #123657

   Purpose

   DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Insert

   mCherry-P2A-DD-AcrIIA4
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   mCherry-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and mU6

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. XLone-Puro Cas13d P2A eGFP-NLS

   Plasmid

   #155182

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   CasRx
   

   Use

   Unspecified; Piggybac transposon

   Tags

   NLS and P2A eGFP NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-MKI-MUT-P2A-GFP-DOX-N

    Plasmid

    #229408

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MKI-MUT-P2A-GFP
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    interaction mutant L109G; L143G (Control mutant)

    Promoter

    TRE3G

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTetOne-human cGASdeltaN-HA

    Plasmid

    #186897

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTetOne-orangutan cGASdeltaN-HA

    Plasmid

    #186898

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Synthetic, Pongo abelii)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTRE-BI-hGRAD

    Plasmid

    #207837

    Purpose

    Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    mCherry
    hFBXW11 (FBXW11 Human)
    VHH-GFP4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    215 amino acids of the full length protein

    Promoter

    TRE3G BI promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro

    Plasmid

    #231972

    Purpose

    Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

    Plasmid

    #204146

    Purpose

    Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Inserts

    SARS-CoV-2 BA.1 variant spike
    zsGreen-T2A-PuR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV and TRE3G

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-DmrB-Casp8-2A-mCherry-puro

    Plasmid

    #231971

    Purpose

    Tet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Casp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTetOne-FLAG-human cGASdeltaN-HA

    Plasmid

    #186899

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro

    Plasmid

    #231973

    Purpose

    Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid

    #155189

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    beta-catenin (Ctnnb1 Mouse)
    

    Use

    Unspecified; Piggybac transposon

    Tags

    P2A eGFP NLS

    Expression

    Mammalian

    Mutation

    Sites phosphorylated by GSK-3beta have been mutat…

    Promoter

    TRE3G

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX MLKL(wt)--2A-mCherry-puro

    Plasmid

    #231974

    Purpose

    Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge