Addgene

We narrowed to 11,728 results for: cel.2;

Showing: 481 - 500 of 11728 results

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pRSF-KpDcr1deltaC(E224Q, GiVL-2)

Plasmid

#32035

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

E224Q and N195-M215 replaced by P697-D714 of GiDi…

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5-2 (w234-1)

Plasmid

#17426

Purpose

Gateway entry vector for N-term V5 tag, frame B

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pFastBac1 Flag Su(Z)2

Plasmid

#20202

Depositor

Nicole Francis

Article

Lo et al Mol Cell Biol. 2009 Jan . 29(2):515-25.

Insert

Su(Z)2 (Su(z)2 Fly)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBABE puro PPAR gamma 2

Plasmid

#8859

Depositor

Bruce Spiegelman

Article

Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

Insert

PPAR gamma (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO.1-MKL1/2 shRNA

Plasmid

#27161

Purpose

Lentiviral shRNA vector for knockdown of both mouse MKL1 and MKL2.

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

shRNA MKL1+2

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-nMyc-wt_3'UTR

Plasmid

#31858

Depositor

Robert Blelloch

Article

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc wild-type 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGL3-PM-34a-K1/2

Plasmid

#25800

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

alternative hsa-miR-34a promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
Axin GID 1-2/pCS2MT

Plasmid

#16299

Depositor

Peter Klein

Article

Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

Insert

Axin GID 1-2 (axin1.L Frog)

Use

Vertebrate expression

Tags

5x myc

Expression

Mutation

GSK interaction domain aa 320-429

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Sal4-wt_3'UTR

Plasmid

#31862

Depositor

Robert Blelloch

Article

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

Sal4 wild-type 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
Axin GID 1-2/pET29b

Plasmid

#16307

Depositor

Peter Klein

Article

Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

Insert

Axin GID 1-2 (axin1.L Frog)

Use

Tags

His

Expression

Bacterial

Mutation

Amino acids 320-429

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Myc-His(-)-HSulf-2

Plasmid

#13004

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 2 (SULF2 Human)

Use

Tags

6x His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-MSulf-2

Plasmid

#13008

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

mouse sulfatase 2 (Sulf2 Mouse)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pQCXIZ CMV/TO DEST (w321-2)

Plasmid

#17401

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-nMyc-m2_3'UTR

Plasmid

#31860

Depositor

Robert Blelloch

Article

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc mutant-1 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Second Let-7c Seed changed from TACCTC to TAACGC

Promoter

SV40

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pX330-H11r1-2-DD-Cas9

Plasmid

#85978

Purpose

Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP nLAP -2

Plasmid

#194322

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro BFP nLAP -2

Plasmid

#194325

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro BFP nLAP +2

Plasmid

#194319

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP nLAP +2

Plasmid

#194307

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pPD187 Tri-AND Gate, 2 repeats

Plasmid

#138940

Purpose

EYFP reporter vector for the Logic 37 AND 43 AND 158 2 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 43 AND 158 2 repeats

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Logic 37 AND 43 AND 158 2 repeats

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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