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  2. pGEX6P1-N-FLAG

    Plasmid
    #119759
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Tags
    FLAG and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119759-plasmid-map-sequence-id-233663

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  5. pLKO_HA.V5.FLAG_NGFR

    Plasmid
    #158250
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158250-plasmid-map-sequence-id-327836

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Showing: 481 - 500 of 13546 results