We narrowed to 16,568 results for: LEA

1. pEN-W-A-ccdB-G-X

   Plasmid

   #218891

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-W-ccdB-Z

   Plasmid

   #218890

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-Myc-Caspase-11_humanized-mutant

   Plasmid

   #214316

   Purpose

   Expression of gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (Casp4 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Mutation

   KLS288YKT_KASIHS348TPRAKA

   Promoter

   MESV

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pITESceIcmd

   Plasmid

   #218288

   Purpose

   Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   HO(-253, -1)-tSynth3

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-C+F-ArchT-GFP

   Plasmid

   #209700

   Purpose

   Expresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinases

   Depositor

   Peer Wulff

   Article

   Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

   Insert

   ArchT-GFP
   

   Use

   AAV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV/CMV-SCLM

   Plasmid

   #216758

   Purpose

   AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon

   Depositor

   George Augustine

   Insert

   SuperClomeleon followed by WPRE and human growth hormone polyA terminator
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   CMV (cytomegalovirus)

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAT_41BBICD

   Plasmid

   #197097

   Purpose

   This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

   Depositor

   Wendell Lim

   Article

   Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

   Insert

   This plasmid contains the coding sequence for the intracellular domain of 4-1BB.
   

   Expression

   Mammalian

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAT_CD28ICD

   Plasmid

   #197098

   Purpose

   This plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

   Depositor

   Wendell Lim

   Article

   Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

   Insert

   This plasmid contains the coding sequence for the intracellular domain of CD28.
   

   Expression

   Mammalian

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-Myc-Caspase-4_R269D

   Plasmid

   #214310

   Purpose

   Expression of gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Mutation

   R269D

   Promoter

   MESV

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-Myc-Caspase-4_K356D

    Plasmid

    #214309

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Mutation

    K356D

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRE-tdTomato

    Plasmid

    #210512

    Purpose

    tdTomato reporter vector for LAUNCHER system

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    tdTomato
    

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY1935

    Plasmid

    #212315

    Purpose

    Express ApmFnuc protein with sumotag

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    Isvmimi
    

    Tags

    6xHis-thrombin site-TwinStrepTag-SUMO

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid

    #202586

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Arginine 261 to Alanine

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid

    #202588

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, changed O…

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnEA-NvH-CtPAN3_448-555_Y

    Plasmid

    #148093

    Purpose

    Bacterial Expression of CtPAN3_448-555

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    CtPAN3_448-555
    

    Expression

    Bacterial

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX SYT9 C2A

    Plasmid

    #195707

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 124-211 of mouse SYT9

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX SYT9 C2B

    Plasmid

    #195708

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 253-344 of mouse SYT9

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX SYT9 C2AB

    Plasmid

    #195709

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 104-386 of mouse SYT9

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX SYT1 C2A

    Plasmid

    #195710

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt1 (Syt1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 96-264 of mouse SYT1

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX SYT1 C2B

    Plasmid

    #195711

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt1 (Syt1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 248-421 of mouse SYT1

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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