We narrowed to 24,999 results for: Nes

1. LMPd Amt PHGDH

   Plasmid

   #209409

   Purpose

   Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

   Depositor

   Russell Jones

   Article

   Ma et al Immunity. 2019 Nov 19;51(5):856-870.e5.

   Insert

   Phgdh shRNA (Phgdh Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSN-A112C-1A32

   Plasmid

   #191241

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Use

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available Since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSN-A112C-1A31

   Plasmid

   #191242

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Use

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSN-A112C-CP8B1

   Plasmid

   #191240

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Use

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Bxb1Donor_G315T_ADRB2_LucReporter

   Plasmid

   #129793

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   G315T

   Promoter

   N/A

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1Donor_W99A_ADRB2_LucReporter

   Plasmid

   #129789

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   W99A

   Promoter

   N/A

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1Donor_N51L_ADRB2_LucReporter

   Plasmid

   #129790

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   N51L

   Promoter

   N/A

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CSKP_HUMAN_D0

   Plasmid

   #79714

   Purpose

   This plasmid encodes the kinase domain of CSKP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSKP (CASK Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pBabe 3XFLAG-T34A-GATA6-3XAU1 puro

   Plasmid

   #72609

   Purpose

   For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutation

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Retroviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Mutation

   changed threonine34 to alanine

   Promoter

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pBabe 3XFLAG-S33A-GATA6-3XAU1 puro

    Plasmid

    #72610

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine33 to alanine

    Promoter

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pEN_TT 3XFLAG-S37A-GATA6-3XAU1

    Plasmid

    #72613

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Mutation

    Changed serine37 to alanine

    Promoter

    TRE tight promoter

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-M-4F2

    Plasmid

    #73431

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 4F2 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pdCas9-M-1B6

    Plasmid

    #73430

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 1B6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pdCas9-M-3H5

    Plasmid

    #73429

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 3H5 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pdCas9-M-1D4

    Plasmid

    #73433

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 1D4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pdCas9-M-1E4

    Plasmid

    #73436

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 1E4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pdCas9-M-5F5

    Plasmid

    #73432

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 5F5 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

    Plasmid

    #72611

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine33, threonine34 and serine37 to ala…

    Promoter

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

19. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220735

    Purpose

    AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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