We narrowed to 15,356 results for: nts

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p2T-CMV-BE4maxCP1028-BlastR

Plasmid

#152993

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9 CP1028-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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PEP115E

Plasmid

#97401

Purpose

binary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Golgi target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:S10-(MCS)

Plasmid

#108177

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUb RxCas13d + RxCas13d guide RNA

Plasmid

#176303

Purpose

Expresses RxCas13d and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

RxCas13d

Tags

HA tag

Expression

Insect

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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PEP116E

Plasmid

#97402

Purpose

binary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Plasma membrane target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

Plasmid

#108260

Purpose

Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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RCP83

Plasmid

#163466

Purpose

Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

Depositor

Frank Albert

Article

Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

Inserts

Promoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP

Expression

Bacterial and Yeast

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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WasCFP /pQE-30

Plasmid

#79858

Purpose

Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00

Insert

WasCFP

Tags

HisTag

Expression

Bacterial

Mutation

Cyan fluorescent protein mCerulean with substitut…

Promoter

T5 promoter / lac operator

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pmNFH

Plasmid

#85178

Purpose

Mouse neurofilament protein H cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Colakoglu et al J Cell Biol. 2009 Jun 1;185(5):769-77. d

Insert

mouse NFH (Nefh Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pEB2_mScarletI_mVenusNB-MrkH

Plasmid

#182291

Purpose

expresses c-di-GMP biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkH

Tags

mVenusNB internal fusion to MrkH

Expression

Bacterial

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRRH

Plasmid

#136924

Purpose

Expresses Cas9-NRRH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRRH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Bgal no NLS

Plasmid

#188399

Purpose

Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-SLITRK3

Plasmid

#82539

Purpose

Lentiviral vector for expressing HRP-SLITRK3 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

SLITRK3 (SLITRK3 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_A1511

Plasmid

#91037

Purpose

Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Promoter

OsAct1

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only
pFastBac_human-IL-18

Plasmid

#214338

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTRANS_213

Plasmid

#91112

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pBPhsFlp2::PEST

Plasmid

#63171

Purpose

Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

hsFlp2::PEST

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-CDAmax-BlastR

Plasmid

#152995

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

CDA-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE655

Plasmid

#153253

Purpose

M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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