We narrowed to 22,212 results for: EGF

1. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

   Plasmid

   #139991

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VRER=D1135V/G1218R/R1335E/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-Enh eGFP Reporter-muKC2-X11

   Plasmid

   #59235

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   Krt7-ex2
   

   Use

   Lentiviral

   Promoter

   Mu Hsp68 minimal promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CAG-Archon1-KGC-EGFP-ER2

   Plasmid

   #108420

   Purpose

   AAV production plasmid encoding for Archon1 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

   Plasmid

   #139484

   Purpose

   PCR template for mEGFP gene knock-in donor vector

   Depositor

   Kazuhiro Aoki

   Article

   Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

   Insert

   mEGFP-loxP-P2A-dTKneo-pA-loxP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

   Plasmid

   #133025

   Purpose

   Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Monoamine oxidase type A (MAOA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-488

   Promoter

   Human elongation factor 1 alpha

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

   Plasmid

   #51656

   Purpose

   hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

   Depositor

   Adam Cohen

   Insert

   Quasar2-mOrange-Chr64-GFP
   

   Use

   Lentiviral

   Tags

   GFP and mOrange

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

   Plasmid

   #122966

   Purpose

   Expresses 3XFlag-tagged human CEP164 in mammalian cells

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Insert

   CEP164 (CEP164 Human)
   

   Use

   Lentiviral

   Tags

   3XFlag

   Promoter

   EF1 alpha

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

   Plasmid

   #134865

   Purpose

   Expresses EGFP-tagged Golgi apparatus marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Membrin (GOSR2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV pTRE-FLEX-EGFP-WPRE-bGHpA

   Plasmid

   #65449

   Purpose

   Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   tetO

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

    Plasmid

    #60814

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Tags

    eGFP-NLS (SV40)

    Expression

    Mammalian

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR]

    Plasmid

    #86449

    Purpose

    pBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid)

    Depositor

    Gregor Bucher

    Insert

    3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR

    Plasmid

    #24378

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::p150yeGFP-DHFR
    

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Available Since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

    Plasmid

    #58959

    Purpose

    lentiviral expression of v-H-Ras and luciferase/EGFP reporter

    Depositor

    Snorri Thorgeirsson

    Article

    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

    Inserts

    CMV enhancer/chicken beta actin promoter
    v-H-Ras
    luc2/EGFP fusion gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV enhancer/chicken beta actin

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

    Plasmid

    #134869

    Purpose

    Expresses EGFP-tagged endoplasmic reticulum marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    cytochrome b5 type A (CYB5A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-98

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. UbC-TRE-mSOD2-tTS-IRES-EGFP

    Plasmid

    #12390

    Depositor

    Zuoshang Xu

    Article

    Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

    Insert

    mSOD2 shRNA (Sod2 Mouse)
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

    Plasmid

    #123614

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A)-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

    Plasmid

    #129708

    Purpose

    Express cre-dependent KASH-EGFP and scramble shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid

    #139995

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    HF1=N497A/R661A/Q695A/Q926A

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC-attB-burs-mCD8-EGFP-T2A-Gal4

    Plasmid

    #39463

    Depositor

    Benjamin White

    Article

    Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

    Insert

    burs-mCD8-EGFP-T2A-Gal4
    

    Promoter

    bursalpha

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

    Plasmid

    #129706

    Purpose

    Express cre-dependent KASH-EGFP and Gmeb1 shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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